Silva Vinicius A O P, Fernandes-Junior Wilson S, Rocha Diego P, Stefano Jéssica S, Munoz Rodrigo A A, Bonacin Juliano A, Janegitz Bruno C
Department of Nature Sciences, Mathematics and Education, Federal University of São Carlos, 13600-970, Araras, São Paulo, Brazil.
Institute of Chemistry, Federal University of Uberlândia, 38400-902, Uberlândia, MG, Brazil.
Biosens Bioelectron. 2020 Dec 15;170:112684. doi: 10.1016/j.bios.2020.112684. Epub 2020 Oct 8.
This work presents a novel procedure involving the sequential chemical treatment to generate reduced graphene oxide (rGO) within 3D-printed polylactic acid (PLA) electrodes and their potential applications for sensing and biosensing. A new configuration of a compact all-3D-printed electrochemical device containing the three electrodes is presented, in which the working electrode was treated to generate rGO within PLA (rGO-PLA) after treatment within NaBH. The rGO-PLA electrodes presented a notable current increase for the redox probe ferrocene-methanol in comparison with the same surface treated by dimethylformamide immersion. Also, the electrochemical impedance spectroscopic data that presented the lowest resistance to electron transfer for the proposed electrode. The electrochemical experiments were in accordance with Raman spectra and surface roughness obtained by atomic force microscopy images. As proofs-of-concept, the rGO-PLA electrode was applied for serotonin determination in synthetic urine using differential-pulse voltammetry with a limit of detection of 0.032 μmol L. Also, the second application involved the fabrication of a tyrosinase-based biosensor capable of determining catechol in natural water samples with a limit of detection of 0.26 μmol L. Based on both applications, the 3D-printed rGO-PLA showed to be an excellent platform for sensing and biosensing purposes.
这项工作提出了一种新颖的方法，该方法涉及顺序化学处理，以在3D打印的聚乳酸（PLA）电极中生成还原氧化石墨烯（rGO）及其在传感和生物传感方面的潜在应用。本文展示了一种包含三个电极的紧凑型全3D打印电化学装置的新配置，其中工作电极在硼氢化钠处理后在PLA（rGO-PLA）中进行处理以生成rGO。与通过二甲基甲酰胺浸渍处理的相同表面相比，rGO-PLA电极对氧化还原探针二茂铁-甲醇的电流有显著增加。此外，电化学阻抗谱数据表明所提出的电极对电子转移的电阻最低。电化学实验与通过原子力显微镜图像获得的拉曼光谱和表面粗糙度一致。作为概念验证，rGO-PLA电极用于使用差分脉冲伏安法测定合成尿液中的血清素，检测限为0.032 μmol/L。此外第二个应用涉及制造一种基于酪氨酸酶的生物传感器，该传感器能够测定天然水样中的儿茶酚，检测限为0.26 μmol/L。基于这两个应用，3D打印的rGO-PLA被证明是用于传感和生物传感目的的优秀平台。
