Center for Advanced Functional Nanorobots, Department of Inorganic Chemistry, University of Chemistry and Technology Prague, Technická 5, 166 28, Prague 6, Czech Republic.
Center for Advanced Functional Nanorobots, Department of Inorganic Chemistry, University of Chemistry and Technology Prague, Technická 5, 166 28, Prague 6, Czech Republic; Department of Chemical and Biomolecular Engineering, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul, 03722, South Korea; Department of Medical Research, China Medical University Hospital, China Medical University, No. 91 Hsueh-Shih Road, Taichung, Taiwan; Future Energy and Innovation Laboratory, Central European Institute of Technology, Brno University of Technology, Purkyňova 656/123, Brno, CZ-616 00, Czech Republic.
Biosens Bioelectron. 2020 Mar 1;151:111980. doi: 10.1016/j.bios.2019.111980. Epub 2019 Dec 23.
Three-dimensional (3D) printing technology offers attractive possibilities for many fields. In electrochemistry, 3D printing technology has been used to fabricate customized 3D-printed electrodes as a platform to develop bio/sensing, energy generation and storage devices. Here, we use a 3D-printed graphene/polylactic (PLA) electrode made by additive manufacturing technology and immobilize horseradish peroxidase (HRP) to create a direct electron transfer enzyme-based biosensors for hydrogen peroxide detection. Gold nanoparticles are included in the system to confirm and facilitate heterogeneous electron transfer. This work opens a new direction for the fabrication of third-generation electrochemical biosensors using 3D printing technology, with implications for applications in the environmental and biomedical fields.
三维（3D）打印技术为许多领域提供了极具吸引力的可能性。在电化学领域，3D 打印技术已被用于制造定制的 3D 打印电极，作为开发生物/传感、能源产生和存储设备的平台。在这里，我们使用通过增材制造技术制造的 3D 打印石墨烯/聚乳酸（PLA）电极，并固定辣根过氧化物酶（HRP）以创建基于直接电子转移酶的生物传感器，用于检测过氧化氢。该系统中包含金纳米粒子，以确认和促进异相电子转移。这项工作为使用 3D 打印技术制造第三代电化学生物传感器开辟了新的方向，对环境和生物医学领域的应用具有重要意义。
