Eckes Stefanie, Braun Joy, Wack Julia S, Ritz Ulrike, Nickel Daniela, Schmitz Katja
Clemens-Schöpf-Institute of Organic Chemistry and Biochemistry, Technical University of Darmstadt, Alarich-Weiss-Straße 8, 64287 Darmstadt, Germany.
Department of Orthopaedics and Traumatology, BiomaTiCS, University Medical Center, Johannes Gutenberg University, Langenbeckstraße 1, 55131 Mainz, Germany.
Int J Mol Sci. 2020 Oct 8;21(19):7408. doi: 10.3390/ijms21197408.
For medical application, easily accessible biomaterials with tailored properties are desirable. Collagen type I represents a biomaterial of choice for regenerative medicine and tissue engineering. Here, we present a simple method to modify the properties of collagen and to generate collagen laminates. We selected three commercially available collagen sheets with different thicknesses and densities and examined the effect of rose bengal and green light collagen crosslinking (RGX) on properties such as microstructure, swelling degree, mechanical stability, cell compatibility and drug release. The highest impact of RGX was measured for Atelocollagen, for which the swelling degree was reduced from 630% (w/w) to 520% (w/w) and thickness measured under force application increased from 0.014 mm to 0.455 mm, indicating a significant increase in mechanical stability. Microstructural analysis revealed that the sponge-like structure was replaced by a fibrous structure. While the initial burst effect during vancomycin release was not influenced by crosslinking, RGX increased cell proliferation on sheets of Atelocollagen and on Collagen Solutions. We furthermore demonstrate that RGX can be used to covalently attach different sheets to create materials with combined properties, making the modification and combination of readily available sheets with RGX an attractive approach for clinical application.
对于医学应用而言，需要易于获取且具有定制属性的生物材料。I型胶原蛋白是再生医学和组织工程的首选生物材料。在此，我们展示了一种简单的方法来改变胶原蛋白的属性并制备胶原蛋白层压板。我们选择了三种具有不同厚度和密度的市售胶原片，并研究了孟加拉玫瑰红和绿光胶原蛋白交联（RGX）对诸如微观结构、溶胀度、机械稳定性、细胞相容性和药物释放等属性的影响。对于去端肽胶原蛋白，RGX的影响最为显著，其溶胀度从630%（w/w）降至520%（w/w），在施加力的情况下测量的厚度从0.014毫米增加到0.455毫米，表明机械稳定性显著提高。微观结构分析显示，海绵状结构被纤维状结构所取代。虽然万古霉素释放过程中的初始突释效应不受交联影响，但RGX增加了去端肽胶原蛋白片和胶原蛋白溶液片上的细胞增殖。我们还证明，RGX可用于共价连接不同的片材以制备具有组合属性的材料，使得利用RGX对现成的片材进行改性和组合成为一种有吸引力的临床应用方法。
