Huang Meng-Meng, Guo Li-Li, Wu Yue-Hong, Lai Qi-Liang, Shao Zong-Ze, Wang Chun-Sheng, Wu Min, Xu Xue-Wei
Key laboratory of Marine Ecosystem and Biogeochemistry, Second Institute of Oceanography, State Oceanic Administration, Hangzhou 310012, PR China.
College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, PR China.
Int J Syst Evol Microbiol. 2018 Jan;68(1):409-415. doi: 10.1099/ijsem.0.002521. Epub 2017 Dec 8.
A Gram-stain-negative, rod-shaped bacterium, designated 157, was isolated from seawater collected from the Philippine Sea. Cells of strain 157 grew in medium containing 0.5-10.0 % NaCl (w/v, optimum 3 %), at pH 6.0-8.5 (optimum 7.0) and at 15-40 °C (optimum 30 °C). Tweens 20, 40 and 80 as well as urea were hydrolysed. The 16S rRNA gene sequence of strain 157 had a high sequence similarity with respect to Pseudooceanicola marinus AZO-C (97.2 %), and exhibited less than 97.0 % sequence similarity to other type strains of the species with validly published names. Phylogenetic analyses revealed that strain 157 fell within a cluster comprising the Pseudooceanicola species and formed a coherent clade with P. marinus AZO-C and Pseudooceanicola antarcticus Ar-45. Strain 157 exhibited average nucleotide identity values of 74.5 and 74.9 % to P. marinus LMG 23705 and P. antarcticus Ar-45, respectively. In silico DNA-DNA hybridization analysis revealed that strain 157 shared 20.2 % DNA relatedness with P. marinus LMG 23705 and 20.6 % with P. antarcticus Ar-45, respectively. The sole isoprenoid quinone was ubiquinone 10. The major fatty acids were summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c), C19 : 0 cyclo ω8c, C16 : 0 2-OH and C16 : 0. The major polar lipids were phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, one unidentified aminolipid and one unidentified glycolipid. The DNA G+C content was 64.6 mol%. According to the phylogenetic, chemotaxonomic and phenotypic data, it represents a novel species of the genus Pseudooceanicola, for which the name Pseudooceanicolalipolyticus is proposed. The type strain is 157 (=KCTC 52654=MCCC 1K03317). In addition, the description of the genus Pseudooceanicola is emended and Oceanicola flagellatus is reclassified as Pseudooceanicola flagellatus comb. nov., with the type strain DY470 (=CGMCC 1.12664=LMG 27871) proposed.
从菲律宾海采集的海水中分离出一株革兰氏阴性、杆状细菌，编号为157。菌株157的细胞能在含0.5 - 10.0%（w/v，最适3%）NaCl、pH 6.0 - 8.5（最适7.0）及15 - 40℃（最适30℃）的培养基中生长。吐温20、40和80以及尿素可被水解。菌株157的16S rRNA基因序列与海假海洋杆菌AZO - C具有较高的序列相似性（97.2%），与其他有效发表名称的该属模式菌株的序列相似性低于97.0%。系统发育分析表明，菌株157属于包含假海洋杆菌属的一个聚类，并与海假海洋杆菌AZO - C和南极假海洋杆菌Ar - 45形成一个连贯的进化枝。菌株157与海假海洋杆菌LMG 23705和南极假海洋杆菌Ar - 45的平均核苷酸同一性值分别为74.5%和74.9%。电子DNA - DNA杂交分析表明，菌株157与海假海洋杆菌LMG 23705的DNA相关性为20.2%，与南极假海洋杆菌Ar - 45的DNA相关性为20.6%。唯一的类异戊二烯醌为泛醌10。主要脂肪酸为总和特征8（C18 : 1ω7c和/或C18 : 1ω6c）、C19 : 0环ω8c、C16 : 0 2 - OH和C16 : 0。主要极性脂为磷脂酰胆碱、磷脂酰乙醇胺、磷脂酰甘油、一种未鉴定的氨基脂和一种未鉴定的糖脂。DNA G + C含量为64.6 mol%。根据系统发育、化学分类和表型数据，它代表假海洋杆菌属的一个新物种，为此提出名称解脂假海洋杆菌。模式菌株为157（=KCTC 52654 = MCCC 1K03317）。此外，对假海洋杆菌属的描述进行了修订，海洋鞭毛杆菌被重新分类为鞭毛假海洋杆菌，提出模式菌株DY470（=CGMCC 1.
