CSIRO Agriculture and Food, 306 Carmody Rd, St Lucia, QLD 4067, Australia.
Australian Research Council Centre of Excellence for Innovations in Peptide and Protein Science, School of Science, Edith Cowan University, Joondalup, WA 6027, Australia.
Int J Mol Sci. 2020 Oct 12;21(20):7512. doi: 10.3390/ijms21207512.
The success of seed germination and the successful establishment of seedlings across diverse environmental conditions depends on seed vigour, which is of both economic and ecologic importance. The smoke-derived exogenous compound karrikins (KARs) and the endogenous plant hormone strigolactone (SL) are two classes of butanolide-containing molecules that follow highly similar signalling pathways to control diverse biological activities in plants. Unravelling the precise mode-of-action of these two classes of molecules in model species has been a key research objective. However, the specific and dynamic expression of biomolecules upon stimulation by these signalling molecules remains largely unknown. Genomic and post-genomic profiling approaches have enabled mining and association studies across the vast genetic diversity and phenotypic plasticity. Here, we review the background of smoke-assisted germination and vigour and the current knowledge of how plants perceive KAR and SL signalling and initiate the crosstalk with the germination-associated hormone pathways. The recent advancement of 'multi-omics' applications are discussed in the context of KAR signalling and with relevance to their adoption for superior agronomic trait development. The remaining challenges and future opportunities for integrating multi-omics datasets associated with their application in KAR-dependent seed germination and abiotic stress tolerance are also discussed.
种子萌发的成功和幼苗在不同环境条件下的成功建立取决于种子活力，这对经济和生态都很重要。源自烟雾的外源化合物卡瑞林（KAR）和内源性植物激素独脚金内酯（SL）是两类含有丁内酯的分子，它们遵循高度相似的信号通路，控制植物中的多种生物活性。揭示这两类分子在模式物种中的精确作用模式一直是一个关键的研究目标。然而，这些信号分子刺激下生物分子的特定和动态表达在很大程度上仍然未知。基因组和后基因组分析方法已经能够挖掘和关联研究巨大的遗传多样性和表型可塑性。在这里，我们回顾了烟雾辅助萌发和活力的背景，以及植物如何感知 KAR 和 SL 信号并启动与萌发相关的激素途径的交叉对话的最新知识。我们还讨论了“多组学”应用的最新进展，以及它们在 KAR 信号中的应用及其与优良农艺性状发育的相关性。还讨论了与 KAR 依赖性种子萌发和非生物胁迫耐受性相关的多组学数据集的整合及其应用的剩余挑战和未来机遇。
