Murashita Yusuke, Nishiuchi Takumi, Rehman Shafiq Ur, Komatsu Setsuko
Faculty of Environment and Information Sciences, Fukui University of Technology, Fukui 910-8505, Japan.
Research Center for Experimental Modeling of Human Disease, Kanazawa University, Kanazawa 920-8640, Japan.
Proteomes. 2021 Oct 2;9(4):39. doi: 10.3390/proteomes9040039.
Plant-derived smoke solution enhances soybean root growth; however, its mechanism is not clearly understood. Subcellular proteomics techniques were used for underlying roles of plant-derived smoke solution on soybean root growth. The fractions of membrane and nucleus were purified and evaluated for purity. ATPase and histone were enriched in the fractions of membrane and nucleus, respectively. Principal component analysis of proteomic results indicated that the plant-derived smoke solution affected the proteins in the membrane and nucleus. The proteins in the membrane and nucleus mainly increased and decreased, respectively, by the treatment of plant-derived smoke solution compared with control. In the proteins in the plasma membrane, ATPase increased, which was confirmed by immunoblot analysis, and ATP contents increased through the treatment of plant-derived smoke solution. Additionally, although the nuclear proteins mainly decreased, the expression of RNA polymerase II was up-regulated through the treatment of plant-derived smoke solution. These results indicate that plant-derived smoke solution enhanced soybean root growth through the transcriptional promotion with RNA polymerase II expression and the energy production with ATPase accumulation.
植物源烟雾溶液可促进大豆根系生长，但其作用机制尚不清楚。采用亚细胞蛋白质组学技术研究植物源烟雾溶液对大豆根系生长的潜在作用。对膜和细胞核组分进行了纯化并评估了纯度。ATP酶和组蛋白分别在膜和细胞核组分中富集。蛋白质组学结果的主成分分析表明，植物源烟雾溶液影响膜和细胞核中的蛋白质。与对照相比，经植物源烟雾溶液处理后，膜和细胞核中的蛋白质分别主要增加和减少。在质膜蛋白质中，ATP酶增加，免疫印迹分析证实了这一点，并且经植物源烟雾溶液处理后ATP含量增加。此外，虽然核蛋白主要减少，但经植物源烟雾溶液处理后RNA聚合酶II的表达上调。这些结果表明，植物源烟雾溶液通过RNA聚合酶II表达的转录促进和ATP酶积累的能量产生来促进大豆根系生长。
