Faculty of Horticulture and Forestry, Banat' s University of Agriculture Science and Veterinary Medicine "King Michael Ist of Romania" from Timisoara, Calea Aradului nr 119, 300645 Timisoara, Romania.
"Coriolan Drăgulescu" Institute of Chemistry, 24 Mihai Viteazul Blvd., 300223 Timisoara, Romania.
Int J Mol Sci. 2021 Mar 10;22(6):2797. doi: 10.3390/ijms22062797.
The phytohormone auxin is involved in almost every process of a plant's life, from germination to plant development. Nowadays, auxin research connects synthetic chemistry, plant biology and computational chemistry in order to develop innovative and safe compounds to be used in sustainable agricultural practice. In this framework, we developed new fluorescent compounds, ethanolammonium -aminobenzoate (HEA-ABA) and -nitrobenzoate (HEA-NBA), and investigated their auxin-like behavior on two main commercial vegetables cultivated in Europe, cucumber () and tomato (), in comparison to the model plant Arabidopsis (). Moreover, the binding modes and affinities of two organic salts in relation to the natural auxin indole-3-acetic acid (IAA) into TIR1 auxin receptor were investigated by computational approaches (homology modeling and molecular docking). Both experimental and theoretical results highlight HEA-ABA as a fluorescent compound with auxin-like activity both in Arabidopsis and the commercial cucumber and tomato. Therefore, alkanolammonium benzoates have a great potential as promising sustainable plant growth stimulators to be efficiently used in vegetable crops.
植物激素生长素几乎参与了植物生命的每一个过程，从萌发到植物发育。如今，生长素研究将合成化学、植物生物学和计算化学联系起来，以开发创新的、安全的化合物，用于可持续的农业实践。在这个框架内，我们开发了新的荧光化合物，乙醇铵-氨基苯甲酸（HEA-ABA）和-硝基苯甲酸（HEA-NBA），并研究了它们在欧洲主要的两种商业蔬菜黄瓜（）和番茄（）上的类似生长素行为，与模式植物拟南芥（）进行了比较。此外，通过计算方法（同源建模和分子对接）研究了两种有机盐与天然生长素吲哚-3-乙酸（IAA）与 TIR1 生长素受体结合模式和亲和力。实验和理论结果都表明，HEA-ABA 是一种具有类似生长素活性的荧光化合物，在拟南芥和商业黄瓜和番茄中均有表现。因此，烷醇铵苯甲酸酯具有作为有前途的可持续植物生长刺激剂的巨大潜力，可有效地用于蔬菜作物。
