Cole E C, Rutala W A, Samsa G P
J Assoc Off Anal Chem. 1987 Jul-Aug;70(4):635-7.
An interlaboratory study was conducted to evaluate a method of standardizing bacterial numbers on penicylinders used in the AOAC use-dilution method (4.007-4.015) of disinfectant testing. Eight participating laboratories followed a broth adjustment method using their media and stock cultures of Staphylococcus aureus ATCC 6538, Salmonella choleraesuis ATCC 10708, and Pseudomonas aeruginosa ATCC 15442. The culture broths that were used to inoculate the penicylinders were incubated for 48 h at 37 degrees C after several (4-6) 24 h passages. McFarland turbidity standards of 1.0 and 0.5 were used to adjust visually the cultures of S. aureus and P. aeruginosa, respectively. S. choleraesuis was used undiluted. The results showed significant variability in numbers of test bacteria which adhered to the penicylinders, with mean values of 1.6 X 10(6) for S. choleraesuis, 3.5 X 10(6) for S. aureus, and 8.2 X 10(6) for P. aeruginosa. The results from collaborating laboratories attempting standardization of bacterial numbers on penicylinders demonstrated significant interlaboratory and cylinder variation for all 3 test organisms.
开展了一项实验室间研究，以评估一种对用于消毒剂测试的AOAC使用稀释法（4.007 - 4.015）中使用的青霉素瓶上的细菌数量进行标准化的方法。八个参与实验室采用肉汤调整法，使用各自的培养基以及金黄色葡萄球菌ATCC 6538、猪霍乱沙门氏菌ATCC 10708和铜绿假单胞菌ATCC 15442的储备培养物。用于接种青霉素瓶的培养肉汤在经过几次（4 - 6次）24小时传代后，于37℃孵育48小时。分别使用麦氏比浊标准1.0和0.5来目视调整金黄色葡萄球菌和铜绿假单胞菌的培养物。猪霍乱沙门氏菌未稀释使用。结果显示，附着在青霉素瓶上的测试细菌数量存在显著差异，猪霍乱沙门氏菌的平均值为1.6×10⁶，金黄色葡萄球菌为3.5×10⁶，铜绿假单胞菌为8.2×10⁶。试图对青霉素瓶上细菌数量进行标准化的协作实验室的结果表明，所有3种测试微生物在实验室间和瓶子之间均存在显著差异。
