Cole E C, Rutala W A
University of North Carolina, Department of Medicine, Chapel Hill 27514.
J Assoc Off Anal Chem. 1988 Jan-Feb;71(1):9-11.
The current AOAC use-dilution methods of disinfectant efficacy testing require the use of 48-54 h unadjusted broth cultures of Salmonella choleraesuis, Staphylococcus aureus, and Pseudomonas aeruginosa for the inoculation of stainless steel penicylinders. The use of unadjusted broth cultures contributes to noncomparable numbers of organisms on penicylinders among the test strains due to relative efficacy of bacterial attachment to penicylinders and to bacterial numbers in broth. To achieve comparable numbers of cells on the penicylinders among the 3 test strains, the cell densities of S. aureus and P. aeruginosa in broth culture were visually adjusted. Growth studies were conducted using S. choleraesuis and P. aeruginosa to determine the numbers of cells in broth at timed intervals and the corresponding numbers of cells attaching to the penicylinders. Results showed that the use of the 24 h broth cultures for all 3 test strains, with adjustment of S. aureus and P. aeruginosa broths, contributes to more comparable numbers of organisms attached to the penicylinders used in disinfectant testing.
当前AOAC用于消毒剂功效测试的使用稀释法要求使用猪霍乱沙门氏菌、金黄色葡萄球菌和铜绿假单胞菌未经调整的48 - 54小时肉汤培养物来接种不锈钢笔形柱。由于细菌附着在笔形柱上的相对功效以及肉汤中的细菌数量，使用未经调整的肉汤培养物会导致各测试菌株在笔形柱上的生物体数量不可比。为了使3种测试菌株在笔形柱上的细胞数量可比，对肉汤培养物中金黄色葡萄球菌和铜绿假单胞菌的细胞密度进行了目测调整。使用猪霍乱沙门氏菌和铜绿假单胞菌进行生长研究，以确定不同时间间隔肉汤中的细胞数量以及附着在笔形柱上的相应细胞数量。结果表明，对所有3种测试菌株使用24小时肉汤培养物，并对金黄色葡萄球菌和铜绿假单胞菌的肉汤进行调整，有助于在消毒剂测试中使用的笔形柱上附着更可比数量的生物体。
