Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, Brazil.
Eur J Oral Sci. 2020 Dec;128(6):495-500. doi: 10.1111/eos.12740. Epub 2020 Oct 15.
The aim of this study was to assess the protective effect of experimental solutions containing chitosan at different viscosities with or without fluoride (TiF /NaF) on dentin loss in vitro. Bovine dentin samples (n = 15) were prepared and allocated to one of the following treatments: (i) 0.5% chitosan (500 mPas); (ii) 0.5% chitosan (2,000 mPas); (iii) 0.042% NaF and 0.049% TiF ; (iv) as (iii) with addition of 0.5% chitosan (500 mPas); (v) as (iii) with addition of 0.5% chitosan (2,000 mPas); (vi) commercial solution with SnCl /AmF/NaF (positive control); or (vii) deionized water (negative control). The samples were submitted to pH cycling for 7 d (0.1% citric acid, 4 × 90 s d ). The treatment was applied once a day for 30 s. The dentin loss was quantified using a contact profilometer. Three samples per group were evaluated using scanning electron microscopy. The dentin loss (μm) was submitted to anova and Tukey's test for differences between treatments. Among the treatments tested, only chitosan 500 mPas was able to statistically significantly reduce the dentin loss compared to the negative control, being similar to the positive control. TiF /NaF, whether with or without chitosan, had no protective effect. Chitosan 500 mPas and SnCl /AmF/NaF solutions have comparable protective effect against dentin erosion in vitro.
本研究旨在评估不同粘度的壳聚糖实验溶液（含或不含氟化物（TiF/NaF））对体外牙本质损耗的保护作用。制备了牛牙本质样本（n=15），并将其分配到以下处理之一：（i）0.5%壳聚糖（500 mPas）；（ii）0.5%壳聚糖（2000 mPas）；（iii）0.042%NaF 和 0.049%TiF；（iv）（iii）中添加 0.5%壳聚糖（500 mPas）；（v）（iii）中添加 0.5%壳聚糖（2000 mPas）；（vi）含 SnCl/AmF/NaF 的商业溶液（阳性对照）；或（vii）去离子水（阴性对照）。样本接受 pH 循环 7 天（0.1%柠檬酸，4×90 s d）。每天处理一次，持续 30 s。使用接触式轮廓仪定量牙本质损耗。每组三个样本用扫描电子显微镜进行评估。牙本质损耗（μm）进行方差分析和 Tukey 检验以比较处理之间的差异。在所测试的处理中，只有 500 mPas 壳聚糖与阴性对照相比，能够显著降低牙本质损耗，与阳性对照相似。TiF/NaF 无论是否含有壳聚糖，都没有保护作用。500 mPas 壳聚糖和 SnCl/AmF/NaF 溶液在体外对牙本质侵蚀具有相当的保护作用。
