Knockdown of Inhibits Papillary Thyroid Carcinoma Cell Invasion and Metastasis by Targeting miR-375.

BACKGROUND

LncRNAs play important roles in papillary thyroid carcinoma (PTC). has been reported to be related to PTC prognosis. The current study aimed to investigate the effects of on human PTC cells.

METHODS

Quantitative real-time polymerase chain reaction (qRT-PCR) was performed to examine expression in PTC tissues and cells and miR-375 expression in PTC cells. Si, miR-375 mimic and miR-375 inhibitor were used for cell transfection. Cell proliferation, apoptosis, migration, and invasion were detected by performing Cell Counting Kit-8 (CCK-8), clone formation assay, flow cytometry, scratch assay, and transwell assay. Western blot was carried out to detect protein levels of E-cadherin, N-cadherin and Snail. The target gene for was verified by dual-luciferase reporter assay.

RESULTS

LINC02471 was highly expressed in PTC tissues and cells. After silencing , cell proliferation, migration and invasion were reduced, but cell apoptosis was increased. Si increased the expressions of E-cadherin and miR-375, and inhibited the expressions of N-Cadherin and Snail. directly targeted miR-375 in PTC cells. Overexpression of miR-375 inhibited the proliferation, migration, invasion of PTC cells and reduced the expressions of N-Cadherin and Snail but promoted the cell apoptosis and increased E-cadherin expression, while miR-375 inhibitor produced opposite effects to overexpressed miR-375. After inhibiting miR-375 expression, si reversed the effect of miR-375 inhibitor.

CONCLUSION

LINC02471 could promote the development of PTC. Knocking down could inhibit invasion and metastasis and promote PTC cell apoptosis through directly targeting miR-375.