Chen Dongfang, Huang Zhongke, Ning Yanli, Lou Cen
Department of Nuclear Medicine, Xiasha Branch of Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, People's Republic of China.
Department of Nuclear Medicine, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, People's Republic of China.
Cancer Manag Res. 2020 Sep 22;12:8757-8771. doi: 10.2147/CMAR.S243767. eCollection 2020.
LncRNAs play important roles in papillary thyroid carcinoma (PTC). has been reported to be related to PTC prognosis. The current study aimed to investigate the effects of on human PTC cells.
Quantitative real-time polymerase chain reaction (qRT-PCR) was performed to examine expression in PTC tissues and cells and miR-375 expression in PTC cells. Si, miR-375 mimic and miR-375 inhibitor were used for cell transfection. Cell proliferation, apoptosis, migration, and invasion were detected by performing Cell Counting Kit-8 (CCK-8), clone formation assay, flow cytometry, scratch assay, and transwell assay. Western blot was carried out to detect protein levels of E-cadherin, N-cadherin and Snail. The target gene for was verified by dual-luciferase reporter assay.
LINC02471 was highly expressed in PTC tissues and cells. After silencing , cell proliferation, migration and invasion were reduced, but cell apoptosis was increased. Si increased the expressions of E-cadherin and miR-375, and inhibited the expressions of N-Cadherin and Snail. directly targeted miR-375 in PTC cells. Overexpression of miR-375 inhibited the proliferation, migration, invasion of PTC cells and reduced the expressions of N-Cadherin and Snail but promoted the cell apoptosis and increased E-cadherin expression, while miR-375 inhibitor produced opposite effects to overexpressed miR-375. After inhibiting miR-375 expression, si reversed the effect of miR-375 inhibitor.
LINC02471 could promote the development of PTC. Knocking down could inhibit invasion and metastasis and promote PTC cell apoptosis through directly targeting miR-375.
长链非编码RNA（lncRNAs）在甲状腺乳头状癌（PTC）中发挥重要作用。已有报道称其与PTC预后相关。本研究旨在探讨[具体内容]对人PTC细胞的影响。
采用定量实时聚合酶链反应（qRT-PCR）检测PTC组织和细胞中[具体内容]的表达以及PTC细胞中miR-375的表达。使用小干扰RNA（Si）、miR-375模拟物和miR-375抑制剂进行细胞转染。通过细胞计数试剂盒-8（CCK-8）、克隆形成试验、流式细胞术、划痕试验和Transwell试验检测细胞增殖、凋亡、迁移和侵袭。进行蛋白质免疫印迹法检测E-钙黏蛋白、N-钙黏蛋白和Snail的蛋白水平。通过双荧光素酶报告基因试验验证[具体内容]的靶基因。
LINC02471在PTC组织和细胞中高表达。沉默[具体内容]后，细胞增殖、迁移和侵袭减少，但细胞凋亡增加。Si增加了E-钙黏蛋白和miR-375的表达，并抑制了N-钙黏蛋白和Snail的表达。[具体内容]在PTC细胞中直接靶向miR-375。miR-375的过表达抑制了PTC细胞的增殖、迁移、侵袭，并降低了N-钙黏蛋白和Snail的表达，但促进了细胞凋亡并增加了E-钙黏蛋白的表达，而miR-375抑制剂产生了与miR-375过表达相反的作用。抑制miR-375表达后，Si逆转了miR-375抑制剂的作用。
LINC02471可促进PTC的发展。敲低[具体内容]可通过直接靶向miR-375抑制侵袭和转移并促进PTC细胞凋亡。
