Derebe Dagninet, Wubetu Muluken, Alamirew Amare
Department of Pharmacy, College of Medicine and Health Sciences, Bahir Dar University, Bahir Dar, Ethiopia.
Department of Pharmacy, College of Health Sciences, Debre Markos University, Debre Markos, Ethiopia.
Clin Pharmacol. 2020 Sep 25;12:149-157. doi: 10.2147/CPAA.S273501. eCollection 2020.
The morbidity and mortality rate from diabetic mellitus are increasing in the world especially in low- and middle-income countries; hence, it is necessary to evaluate the efficacy and safety of medicinal plants to support existing drugs in treating diabetes mellitus. Therefore, the aim of this study was to evaluate the hypoglycemic effect of 80% methanol root extract of in normoglycemic, hyperglycemic, and streptozotocin-nicotinamide induced diabetic rats.
Male albino Wistar rats were divided into five groups (n=6) in all three models. In all models, group one rats served as a negative control and were received vehicle (10mL/kg distilled water), whereas group two (APRE100), three (APRE200), and four (APRE400) were treated with 100, 200, and 400mg/kg of extract, respectively, and group five were treated with glibenclamide (5mg/kg) and served as a positive control. Blood glucose levels were measured at different time points by taking blood from their tails. Data were analyzed using one-way ANOVA followed by Tukey's post hoc test to carry out comparisons between and within-group and P < 0.05 was considered as statistically significant.
The root of reduces peak blood sugar levels significantly after the loading of oral glucose at all tested doses. In streptozotocin-nicotinamide-induced type 2 diabetic rats, the daily oral administration of the crude extracts showed a significant reduction of blood glucose level at all tested doses compared to the negative control group. However, the extract did not reduce blood glucose levels in normoglycemic rats at all tested doses compared to both negative and positive control.
From this study, it can be concluded that the root extract of showed an antihyperglycemic effect in hyperglycemic and diabetic rats but lack hypoglycemic effect in normoglycemic rats. Hence, the plant root may be a good candidate for the development of new antidiabetic drugs.
在全球范围内，尤其是在低收入和中等收入国家，糖尿病的发病率和死亡率正在上升；因此，有必要评估药用植物在辅助现有药物治疗糖尿病方面的疗效和安全性。因此，本研究的目的是评估[植物名称]80%甲醇根提取物对血糖正常、高血糖和链脲佐菌素-烟酰胺诱导的糖尿病大鼠的降血糖作用。
在所有三种模型中，将雄性白化Wistar大鼠分为五组（每组n = 6）。在所有模型中，第一组大鼠作为阴性对照，接受赋形剂（10 mL/kg蒸馏水），而第二组（APRE100）、第三组（APRE200）和第四组（APRE400）分别用100、200和400 mg/kg提取物处理，第五组用格列本脲（5 mg/kg）处理并作为阳性对照。通过从大鼠尾部取血在不同时间点测量血糖水平。使用单因素方差分析，随后进行Tukey事后检验分析数据，以进行组间和组内比较，P < 0.05被认为具有统计学意义。
在所有测试剂量下，口服葡萄糖负荷后，[植物名称]根显著降低血糖峰值水平。在链脲佐菌素-烟酰胺诱导的2型糖尿病大鼠中，与阴性对照组相比，每天口服粗提物在所有测试剂量下均显著降低血糖水平。然而，与阴性和阳性对照相比，在所有测试剂量下，提取物均未降低血糖正常大鼠的血糖水平。
从本研究可以得出结论，[植物名称]根提取物在高血糖和糖尿病大鼠中显示出抗高血糖作用，但在血糖正常大鼠中缺乏降血糖作用。因此，该植物根可能是开发新型抗糖尿病药物的良好候选物。
