Yrga Adugna Baye, Mequanint Adinew Getinet, Ayalew Getahun Kefyalew, Endale Gurmu Abyot, Yirga Berhie Alemshet, Awoke Tewachew, Tessema Desta Getaye
Department of Pharmacy, Bahir Dar Health Science College, Bahir Dar, Ethiopia.
Department of Pharmacology, School of Pharmacy, College of Medicine and Health Sciences, University of Gondar, Gondar, Ethiopia.
Evid Based Complement Alternat Med. 2022 Jun 30;2022:5193250. doi: 10.1155/2022/5193250. eCollection 2022.
Diabetes Mellitus isa chronic metabolic disorder that required long-lasting treatment. In Ethiopian traditional medicine practices, abundant plants have been used for the treatment of diabetes mellitus for a long period. The root of is employed for the treatment of diabetes mellitus by Ethiopians. This study aimed to investigate the antidiabetic activity of the crude extract of root in albino mice.
Cold maceration technique and hydro methanolic (80% methanol) solvent with occasionally shaking were employed during the crude extraction processes. To evaluate the antidiabetic activity of the crude extract, normoglycemic, glucose-loaded, and streptozotocin-induced diabetic models were used. In each model, the overnight fasted mice were randomly divided into five groups for normoglycemic and glucose-loaded models as a negative control, positive control, and three tested groups, whereas, in streptozotocin-induced diabetic models, the mice were grouped into six groups like one diabetic and one normal negative control groups, three diabetic tested groups, and one diabetic positive group. Each group comprised six mice. For all models, the tested groups received the crude extract at 100, 200, and 400 mg/kg doses, both diabetic and nondiabetic negative control groups received 10 ml/kg distilled water, and positive groups received 5 mg/kg glibenclamide.
The crude extract of the plant did not show any sign of toxicity up to 2000 mg/kg dose. In normoglycemic and oral glucose tolerance tests, the crude extract significantly ( < 0.01) reduced the blood glucose level at 200 and 400 mg/kg doses. In the streptozotocin-induced diabetes models, a significant effect was observed at all tested doses.
The finding of this study revealed that the crude extract of the plant owned antidiabetic activity and supports the traditional use of root for the treatment of diabetes mellitus.
糖尿病是一种需要长期治疗的慢性代谢紊乱疾病。在埃塞俄比亚传统医学实践中，长期以来大量植物被用于治疗糖尿病。埃塞俄比亚人使用[植物名称]的根来治疗糖尿病。本研究旨在调查[植物名称]根的粗提物对白化病小鼠的抗糖尿病活性。
在粗提过程中采用冷浸法和氢甲醇（80%甲醇）溶剂，偶尔摇晃。为评估粗提物的抗糖尿病活性，使用了正常血糖、葡萄糖负荷和链脲佐菌素诱导的糖尿病模型。在每个模型中，过夜禁食的小鼠在正常血糖和葡萄糖负荷模型中随机分为五组，分别作为阴性对照、阳性对照和三个测试组，而在链脲佐菌素诱导的糖尿病模型中，小鼠分为六组，即一个糖尿病组和一个正常阴性对照组、三个糖尿病测试组和一个糖尿病阳性组。每组包括六只小鼠。对于所有模型，测试组分别接受100、200和400mg/kg剂量的粗提物，糖尿病和非糖尿病阴性对照组接受10ml/kg蒸馏水，阳性组接受5mg/kg格列本脲。
该植物的粗提物在高达2000mg/kg剂量时未显示出任何毒性迹象。在正常血糖和口服葡萄糖耐量试验中，粗提物在200和400mg/kg剂量时显著（P<0.01）降低了血糖水平。在链脲佐菌素诱导的糖尿病模型中，在所有测试剂量下均观察到显著效果。
本研究结果表明该植物的粗提物具有抗糖尿病活性，支持了[植物名称]根在治疗糖尿病方面的传统用途。
