Alene Molalegn, Abdelwuhab Mohammedbrhan, Belay Assefa, Yazie Taklo Simeneh
Department of Pharmacology, College of Medicine and Health Sciences, University of Gondar, P.O. Box 196, Gondar, Amhara, Ethiopia.
Pharmacology Unit and Research Team, Department of Pharmacy, College of Health Sciences, Debre Tabor University, P.O. Box 272, Debre Tabor, Amhara, Ethiopia.
Evid Based Complement Alternat Med. 2020 Dec 22;2020:6642588. doi: 10.1155/2020/6642588. eCollection 2020.
Traditional healers and the community have used the roots of for the treatment of diabetes mellitus. It is not scientifically validated for its antidiabetic activity previously. Therefore, the objective of the present study was to determine the hypoglycemic and antidiabetic activity of . roots' crude extract and solvent fractions were prepared. The doses of 100 mg/kg, 200 mg/kg, and 400 mg/kg of crude root extract and solvent fractions were used on normoglycemic, oral glucose loaded, and streptozotocin-induced diabetic mice models to determine their hypoglycemic and antihyperglycemic activities. The crude extract and solvent fractions' effect on bodyweight was also evaluated on streptozotocin-induced diabetic mice. A standard drug in all cases was glibenclamide (5 mg/kg), and the blood glucose level was measured by using a glucose meter. Data analysis was performed by using Statistical Package for Social Sciences version 21. One-way analysis of variance followed by Tukey's post hoc multiple comparison test was used to analyze the data. value < 0.05 was considered statistically significant. Hydromethanolic crude extract and its aqueous fraction of root showed a considerable blood glucose lowering activity at all doses. Both the repeated daily doses of the crude extract and the repeated daily doses of the aqueous fraction of root extract revealed the similar effect in lowering the fasting blood glucose level in streptozotocin-induced diabetic mice models. It was also found that groups treated with the at the doses of 200 mg/kg and 400 mg/kg showed significant ( < 0.05) bodyweight improvement at the 14 day of treatment compared to the respective baseline bodyweight, and the diabetic control group showed significant ( < 0.01) reduction in bodyweight at the 14 day compared to the baseline. This study revealed that crude extract and solvent fractions of root possess significant antidiabetic activity which supports its traditional use for the treatment of diabetes mellitus.
传统治疗师和社区一直使用[植物名称]的根来治疗糖尿病。此前其抗糖尿病活性尚未得到科学验证。因此，本研究的目的是确定[植物名称]根的降血糖和抗糖尿病活性。制备了[植物名称]根的粗提物和溶剂提取物。将100mg/kg、200mg/kg和400mg/kg剂量的根粗提物和溶剂提取物用于正常血糖、口服葡萄糖负荷和链脲佐菌素诱导的糖尿病小鼠模型，以确定它们的降血糖和抗高血糖活性。还评估了粗提物和溶剂提取物对链脲佐菌素诱导的糖尿病小鼠体重的影响。所有情况下的标准药物均为格列本脲（5mg/kg），并使用血糖仪测量血糖水平。使用社会科学统计软件包第21版进行数据分析。采用单因素方差分析，随后进行Tukey事后多重比较检验来分析数据。P值<0.05被认为具有统计学意义。[植物名称]根的水甲醇粗提物及其水相部分在所有剂量下均显示出显著的血糖降低活性。粗提物的每日重复剂量和[植物名称]根提取物水相部分的每日重复剂量在降低链脲佐菌素诱导的糖尿病小鼠模型空腹血糖水平方面显示出相似的效果。还发现，与各自的基线体重相比，接受200mg/kg和400mg/kg剂量[植物名称]治疗的组在治疗第14天时体重有显著（P<0.05）改善，而糖尿病对照组在第14天时与基线相比体重有显著（P<0.01）下降。本研究表明，[植物名称]根的粗提物和溶剂提取物具有显著的抗糖尿病活性，这支持了其在治疗糖尿病方面的传统用途。
