Derebe Dagninet, Abdulwuhab Mohammedbrhan, Wubetu Muluken, Mohammed Faiz
Department of Pharmacy, College of Medicine and Health Sciences, Bahir Dar University, Ethiopia.
School of Pharmacy, College of Medicine and Health Sciences, Gondar University, Ethiopia.
Evid Based Complement Alternat Med. 2018 Oct 4;2018:1360486. doi: 10.1155/2018/1360486. eCollection 2018.
Diarrhea is a major health problem throughout the world and it has become more problematic in developing countries like Ethiopia. People, in several parts of the world, use different traditional medicines for treating diarrhea and it has been reported that the roots, leaves, and flowers of various species are used for the same purpose. In Ethiopia, for instance, is used for the treatment of diarrhea and also to control infection. The aim of the present study was, therefore, to evaluate the in vivo antidiarrheal and in vitro antimicrobial effect of in mice. For the antimicrobial activity test, four standard bacteria and disc diffusion method were used, while for antidiarrheal experiment, animals had been used, which were divided into 5 groups. The first group served as negative control and was administered with vehicle (0.2-0.3ml of distilled water). Groups two (D100), three (D200), and four (D400) were administered 100, 200, and 400 mg/kg of the extract, respectively. Group five served as positive control group and was administered with either loperamide (3mg/kg) for castor oil induced diarrhea and castor oil induced enteropooling diarrhea models or atropine (1mg/kg) for charcoal meal test. Safety study was performed using a standard acute toxicity study procedure. The effect of the extract on castor oil induced diarrheal drops, onset of diarrhea, weight of faeces, small intestinal fluid accumulation, and intestinal motility was measured and analyzed using one-way ANOVA. Preliminary phytochemical screening of the leaves powder of the plant indicated the presence of various components. Inhibition of castor oil induced diarrhea was observed at all tested doses. It can be concluded that crude extracts of showed strong activities against diarrhea indicating that it contains some chemical constituents that possibly lead to antidiarrheal drug development.
腹泻是全球主要的健康问题，在埃塞俄比亚等发展中国家，这一问题愈发严重。在世界上的多个地区，人们使用不同的传统药物治疗腹泻，据报道，多种植物的根、叶和花被用于此目的。例如，在埃塞俄比亚，[植物名称]被用于治疗腹泻以及控制感染。因此，本研究的目的是评估[植物名称]在小鼠体内的止泻作用和体外抗菌效果。抗菌活性测试采用了四种标准细菌和纸片扩散法，而止泻实验则使用了动物，这些动物被分为5组。第一组作为阴性对照，给予赋形剂（0.2 - 0.3毫升蒸馏水）。第二组（D100）、第三组（D200）和第四组（D400）分别给予100、200和400毫克/千克的提取物。第五组作为阳性对照组，对于蓖麻油诱导的腹泻和蓖麻油诱导的肠积液腹泻模型给予洛哌丁胺（3毫克/千克），对于炭末试验给予阿托品（1毫克/千克）。使用标准的急性毒性研究程序进行安全性研究。采用单因素方差分析测量并分析提取物对蓖麻油诱导的腹泻次数、腹泻发作时间、粪便重量、小肠液积聚和肠道蠕动的影响。对该植物叶粉进行的初步植物化学筛选表明存在多种成分。在所有测试剂量下均观察到对蓖麻油诱导腹泻的抑制作用。可以得出结论，[植物名称]的粗提物显示出强大的止泻活性，表明其含有一些可能有助于开发止泻药物的化学成分。
