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孢子粉含有少量三萜类化合物。

spore powder contains little triterpenoids.

作者信息

Liu Mei-Ting, Chen Ling-Xiao, Zhao Jing, Li Shao-Ping

机构信息

State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China.

出版信息

Chin Med. 2020 Oct 12;15:111. doi: 10.1186/s13020-020-00391-1. eCollection 2020.

DOI:10.1186/s13020-020-00391-1
PMID:33062047
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7552346/
Abstract

BACKGROUND

spore is a minuscule germ cell ejected from gills during its growth maturity period, it has been considered with high exploitable potential in health-care products manufacture.

METHODS

After testing sporoderm-broken rate, the triterpenoids in 12 batches of broken and unbroken spore powder (GSP) samples were compared with fruiting body (GL) by high performance thin-layer chromatography (HPTLC) and further verified by liquid chromatography coupled with electrospray ionization quadrupole-time-of-flight mass spectrometry (LC-QTOF-MS); meanwhile, the dissolution of triterpenoids after bionic extraction was also investigated by HPTLC.

RESULTS

The sporoderm-broken rate of all the broken GSP samples was over 85%. The relative peak area of triterpenoids in GSP samples were lower than 50% of that in fruiting body, and the dissolution of triterpenoids in artificial gastrointestinal fluid was lower than in methanol.

CONCLUSIONS

This study demonstrated that there were little triterpenoids in GSP. Triterpenoids in GSP also seldom be dissolved in artificial gastrointestinal fluid.

摘要

背景

孢子是在其生长成熟期从菌褶中排出的微小生殖细胞，在保健品制造中被认为具有很高的开发潜力。

方法

在检测破壁率后，通过高效薄层色谱法（HPTLC）对12批破壁和未破壁孢子粉（GSP）样品中的三萜类化合物与子实体（GL）进行比较，并通过液相色谱-电喷雾电离四极杆-飞行时间质谱法（LC-QTOF-MS）进一步验证；同时，还通过HPTLC研究了仿生提取后三萜类化合物的溶出情况。

结果

所有破壁GSP样品的破壁率均超过85%。GSP样品中三萜类化合物的相对峰面积低于子实体的50%，且三萜类化合物在人工胃肠液中的溶出率低于在甲醇中的溶出率。

结论

本研究表明GSP中三萜类化合物含量很少。GSP中的三萜类化合物在人工胃肠液中也很少溶解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f1a/7552346/5ca13478fdd7/13020_2020_391_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f1a/7552346/e4e0714b230c/13020_2020_391_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f1a/7552346/9801b13af7fc/13020_2020_391_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f1a/7552346/5ca13478fdd7/13020_2020_391_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f1a/7552346/e4e0714b230c/13020_2020_391_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f1a/7552346/9801b13af7fc/13020_2020_391_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f1a/7552346/5ca13478fdd7/13020_2020_391_Fig3_HTML.jpg

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