Faculty of Engineering and the Institute of Nanotechnology and Advanced Materials, Bar-Ilan University, Ramat-Gan 5290002, Israel.
Cardiovascular Biology Laboratories at the Felsenstein Medical Research Center and the Cardiology Department, Rabin Medical Center, Petah-Tikva 4941492, Israel.
Nano Lett. 2020 Nov 11;20(11):8360-8368. doi: 10.1021/acs.nanolett.0c03525. Epub 2020 Oct 16.
The strategy of identification for M1 and M2 macrophages both and would help to predict the health condition of the individual. Here, we introduced a solution to this problem with the advantage of both the phagocytic nature of macrophages and the scattering effect of gold nanorods (GNRs). The internalized GNRs, relating to their extent of intake, caused a conspicuous scattering profile at the red channel in flow cytometry, overruling the contribution of the cellular side scatters. This internalization is solely governed by the surface chemistry of GNRs. The PAH-GNRs showed maximum intake potency followed by Cit-, PSS-, and PEG-GNRs. On a substantial note, PAH-GNRs lead to differential uptake between M1 and M2 cells, with three times higher intake in M2 cells over M1. This is the first report of employing the scattering of unlabeled GNRs to discriminate M1 and M2 cell types using a flow cytometer.
M1 和 M2 巨噬细胞的鉴定策略都有助于预测个体的健康状况。在这里，我们提出了一种解决方案，利用巨噬细胞的吞噬特性和金纳米棒（GNRs）的散射效应来解决这个问题。被内化的 GNRs 与其摄入程度有关，在流式细胞术中在红色通道引起明显的散射分布，从而忽略了细胞侧向散射的贡献。这种内化仅受 GNRs 表面化学性质的控制。PAH-GNRs 表现出最大的摄取效力，其次是 Cit-、PSS-和 PEG-GNRs。值得注意的是，PAH-GNRs 导致 M1 和 M2 细胞之间的摄取差异，M2 细胞的摄取量比 M1 细胞高 3 倍。这是首次报道使用未标记的 GNRs 的散射来使用流式细胞仪区分 M1 和 M2 细胞类型。
