Department of Parasitology and Tropical Medicine, Chonnam National University Medical School, Gwangju, Korea.
Department of Laboratory Medicine, Chonnam National University Medical School, Gwangju, Korea.
Ann Lab Med. 2021 Mar 1;41(2):221-224. doi: 10.3343/alm.2021.41.2.221.
Stool examination is the gold standard for the detection of intestinal parasites. We assessed the performance of a newly developed AVE-562 analyzer (AVE Science & Technology Co., Hunan, China) for the vision-based detection of eggs of -the most common intestinal parasite in Korea-in stool samples. In total, 30 stool samples with a high or low egg count or without eggs (as negative control samples) (N=10 each) were prepared and analyzed. The performance of the AVE-562 analyzer was compared with that of the formalin-ether concentration (FEC) method. The overall correct identification rate of the AVE-562 analyzer based on FEC results was 66.6%. The sensitivity, specificity, positive predictive value, and negative predictive value of the AVE-562 analyzer for detecting C. sinensis eggs were 36.4%, 100.0%, 100.0%, and 73.1%, respectively. The average time required to run five tests simultaneously was 27 min using the AVE-562 analyzer and 58 min using the FEC method. Although the AVE-562 analyzer enables rapid and convenient stool examination, its sensitivity needs to be improved, particularly considering the prevalence of low-burden infection in Korea.
粪便检查是检测肠道寄生虫的金标准。我们评估了一种新开发的 AVE-562 分析仪（AVE 科学技术有限公司，湖南，中国）在基于视觉的检测粪便样本中最常见的韩国肠道寄生虫卵方面的性能。总共准备并分析了 30 个具有高或低卵计数或无卵（作为阴性对照样本）（N=10 个）的粪便样本。AVE-562 分析仪的性能与甲醛乙醚浓缩（FEC）方法进行了比较。基于 FEC 结果，AVE-562 分析仪的总体正确识别率为 66.6%。AVE-562 分析仪检测华支睾吸虫卵的灵敏度、特异性、阳性预测值和阴性预测值分别为 36.4%、100.0%、100.0%和 73.1%。使用 AVE-562 分析仪同时运行五次测试的平均时间为 27 分钟，而使用 FEC 方法的平均时间为 58 分钟。尽管 AVE-562 分析仪能够快速方便地进行粪便检查，但它的灵敏度需要提高，特别是考虑到韩国低负担感染的流行率。
