Bioanalysis Scientific Group, Faculty of Pharmacy, Collegium Medicum in Bydgoszcz at Nicolaus Copernicus University in Toruń, dr. Antoniego Jurasza 2 street, 85-089 Bydgoszcz, Poland.
Analyst. 2020 Nov 9;145(22):7279-7288. doi: 10.1039/d0an01379j.
Oral fluid testing is steadily building its position as a valuable complement or alternative to plasma and urine analyses in everyday laboratory practice. However, the great significance of the sample collection process in the attainment of representative results is not always paralleled by the attention given to its informed selection. Few evaluations of commercially available sample collection devices have been published until now, and the current work intends to fill this gap by presenting an evaluation of swabs from 15 different devices for the analysis of 49 popular drugs. Swabs, derived from sample collection devices, were used to collect a drug-fortified mixture. Then, swab-retrieved samples were subjected to instrumental analysis with the high-performance liquid chromatography coupled with tandem mass spectrometry method. Results within the 80-120% range were considered to have no significant impact on analyte concentration (thus satisfactory) and were observed in 44.1% of all results. Out of the 15 evaluated swabs, 7 provided results in the aforementioned range for more than half of the substances under study. The possibility of matrix effects originating from swab materials was also investigated. The selection of an appropriate oral fluid sample collection method plays a critical role in the success of the analytical procedure, a fact that is well-illustrated by the tremendous differences between analyte concentrations observed in this research. Perhaps, the tedious labour of improving sample preparation and analysis methods already in-use could be spared if only greater emphasis were to be put on the improvement and better selection of suitable solutions for oral fluid collection.
口腔液检测在日常实验室实践中作为一种有价值的补充或替代血浆和尿液分析的方法，其地位稳步提升。然而，在获得有代表性的结果时，样本采集过程的重要性并不总是与对其信息采集的重视程度相匹配。直到现在，很少有关于市售样本采集设备的评估发表，而目前的工作旨在通过评估来自 15 种不同设备的拭子来填补这一空白，这些拭子用于分析 49 种常见药物。拭子取自样本采集设备，用于收集加药的混合物。然后，用高效液相色谱-串联质谱法对拭子回收的样本进行仪器分析。在 80-120%范围内的结果被认为对分析物浓度没有显著影响（因此是令人满意的），在所有结果的 44.1%中观察到了这种情况。在所评估的 15 种拭子中，有 7 种对超过一半的研究物质提供了上述范围内的结果。还研究了来自拭子材料的基质效应的可能性。选择适当的口腔液样本采集方法对于分析程序的成功至关重要，这一事实在本研究中观察到的分析物浓度的巨大差异中得到了很好的体现。也许，如果仅仅更加重视改进和更好地选择合适的口腔液采集解决方案，就可以省去改进和分析方法中繁琐的劳动。
