Chen Pan, Pan Liqin, Huang Wensi, Feng Huijuan, Ouyang Wei, Wu Juqing, Wang Jing, Deng Yuying, Luo Jiaxin, Chen Yanying
Department of Nuclear Medicine, Zhujiang Hospital of Southern Medical University, Guangzhou, Guangdong Province, China.
Endocr Connect. 2020 Oct;9(10):999-1008. doi: 10.1530/EC-20-0420.
To evaluate the relationship between the BRAF V600E mutation in lymph node metastasis (LNM) and its invasive characteristics in papillary thyroid cancer (PTC).
A total of 373 PTC patients were enrolled in this study conducted at Zhujiang Hospital of Southern Medical University between January 2017 and December 2018. PTCs with cervical lymph node metastases were verified pathohistologically, and primary tumors and LNM were examined for the BRAF V600E mutation. Patients were excluded from the study if the BRAF V600E mutation was examined only in primary tumors or only in LNM.
Of the 373 patients examined, BRAF V600E mutation frequency in primary tumors was slightly higher than in LNM (81.5% vs 78.0%, P = 0.000), the intra-class correlation coefficient (ICC) was 0.865 (95% CI 0.835-0.890). The BRAF V600E mutation in both primary tumor and LNM negatively correlated with the size of the largest metastatic focus of LNM (Odds ratio, OR = 0.297, 95% CI 0.143-0.616, P = 0.001; OR = 0.242, 95% CI 0.119-0.492, P = 0.000, respectively). There was no relationship between BRAF V600E mutation in LNM and the number, extranodal extension or stage of LNM (P > 0.05).
The BRAF V600E mutation in LNM may not be related to the invasive characteristics of LNM in PTC.
评估甲状腺乳头状癌（PTC）中淋巴结转移（LNM）的BRAF V600E突变与其侵袭特征之间的关系。
2017年1月至2018年12月在南方医科大学珠江医院进行的这项研究共纳入了373例PTC患者。经病理组织学证实为伴有颈部淋巴结转移的PTC，检测原发肿瘤和LNM中的BRAF V600E突变。如果仅在原发肿瘤或仅在LNM中检测BRAF V600E突变，则将患者排除在研究之外。
在373例接受检查的患者中，原发肿瘤的BRAF V600E突变频率略高于LNM（81.5%对78.0%，P = 0.000），组内相关系数（ICC）为0.865（95%CI 0.835 - 0.890）。原发肿瘤和LNM中的BRAF V600E突变均与LNM最大转移灶的大小呈负相关（优势比，OR = 0.297，95%CI 0.143 - 0.616，P = 0.001；OR = 0.242，95%CI 0.119 - 0.492，P = 0.000）。LNM中的BRAF V600E突变与LNM的数量、结外扩展或分期之间无相关性（P > 0.05）。
LNM中的BRAF V600E突变可能与PTC中LNM的侵袭特征无关。
