Doctorado en Ciencias Biológicas y de la Salud, Universidad Autónoma Metropolitana, Ciudad de México, CP 14380, México.
Departamento de Sistemas Biológicos, División de Ciencias Biológicas y de la Salud, Universidad Autónoma Metropolitana-Xochimilco, Ciudad de México, CP 04960, México.
J Dairy Sci. 2020 Dec;103(12):11129-11137. doi: 10.3168/jds.2020-18516. Epub 2020 Oct 15.
Considering the increase in evidence regarding the benefits of probiotics on human health, there is interest in developing solid products with proper functional characteristics, such as temperature and pH stability, that can be added to oral solid dosage forms or to dairy products to release microorganisms directly at their site of action. The aim of this work was to develop a product with an enteric coat containing probiotics that is stable at room temperature and resists low pH to ensure that the probiotics are passed through the stomach and reach the colon. We obtained 2 enteric-release products based on the incorporation of Bifidobacterium sp. using commercial microcrystalline cellulose (BIP-Av) and prebiotic inulin (BIP-In) as cores. Both products had an initial concentration of approximately 1 × 10 bifidobacteria per gram (cfu/g) and showed a suitable resistance to acid; complete release from the products at a pH of 7.5 was observed at 120 min for BIP-In and 180 min for BIP-Av. The viability of bacteria in both products decreased by approximately 3 orders of magnitude. The death rate constant corresponded to 0.1143 for BIP-Av and 0.1466 for BIP-In, which means that in these storage conditions, the viability decreased slightly. Both products protected bifidobacteria for more than 2 yr, delivering a concentration of more than 1 × 10 cfu/g. Due to these characteristics, the products could be incorporated into solid pharmaceutical forms for oral administration. These products could have significant advantages over existing products on the market and provide protection for bacteria, allowing their passage through the stomach to reach the colon, and the viability of bacteria was maintained after storage at room temperature for more than 1 yr.
鉴于越来越多的证据表明益生菌对人类健康有益，人们对开发具有适当功能特性的固体产品（如温度和 pH 值稳定性）产生了兴趣，这些产品可以添加到口服固体制剂或乳制品中，以便在作用部位直接释放微生物。本工作旨在开发一种含有益生菌的肠溶包衣产品，该产品在室温下稳定且能抵抗低 pH 值，以确保益生菌通过胃部到达结肠。我们使用商业微晶纤维素（BIP-Av）和益生元菊粉（BIP-In）作为核心，获得了 2 种基于双歧杆菌属（Bifidobacterium sp.）的肠溶释放产品。这两种产品的初始双歧杆菌浓度约为 1×10 cfu/g，且对酸具有适宜的抗性；在 pH 值为 7.5 时，BIP-In 产品在 120 分钟时、BIP-Av 产品在 180 分钟时完全从产品中释放出来。两种产品中细菌的存活率均降低了约 3 个数量级。细菌的死亡速率常数分别为 0.1143（BIP-Av）和 0.1466（BIP-In），这意味着在这些储存条件下，存活率略有下降。两种产品都能保护双歧杆菌 2 年以上，其浓度均超过 1×10 cfu/g。由于这些特性，这些产品可以被纳入口服固体制剂中。与市场上现有的产品相比，这些产品可能具有显著的优势，可以保护细菌，使其通过胃部到达结肠，并且在室温下储存 1 年以上后，细菌的存活率仍能保持。
