Aires Alfredo, Carvalho Rosa
Centre for the Research and Technology for Agro-Environment and Biological Sciences, CITAB, University of Trás-Os-Montes E Alto Douro, UTAD, Quinta de Prados, 5000-801 Vila Real, Portugal.
Agronomy Department, School of Agrarian and Veterinarian Sciences, University of Trás-Os-Montes E Alto Douro, UTAD, Quinta de Prados, 5000-801 Vila Real, Portugal.
J Food Sci Technol. 2020 Nov;57(11):4265-4276. doi: 10.1007/s13197-020-04466-7. Epub 2020 Apr 28.
A response surface methodology was used to study the conditions for a maximum recovery of phenolics from processing kiwi fruit residues. Ethanolic extracts were prepared with different conditions of pH (2, 5, 10), temperature (30, 50 and 70 ºC) and time (10, 20, 30 min). Total phenolics, total flavonoids content and antioxidant activities by 2,2-diphenyl-1-picrylhydrazyl scavenging capacity and ferric reducing antioxidant power were determined. Samples from optimal extraction condition were injected HPLC-DAD system to access the phenolic profile and content. The best extraction conditions were pH solvent of 2, 70 ºC of temperature and 20 min of extraction. Ten phenolics were identified: caffeic acid and its derivatives, chlorogenic acid and ferulic acid, (+)-catechin, (-)-epicatechin), rutin and quercitrin. These phenolics often reported as having important antioxidant, anti-inflammatory, antiaging and anticancer activities, turn this residues and excellent source of bioactive compounds to be used in agro-food, cosmetics or phytochemical industries.
采用响应面法研究从猕猴桃加工残渣中最大程度回收酚类物质的条件。在不同的pH值(2、5、10)、温度(30、50和70℃)和时间(10、20、30分钟)条件下制备乙醇提取物。通过2,2-二苯基-1-苦基肼清除能力和铁还原抗氧化能力测定总酚、总黄酮含量及抗氧化活性。将最佳提取条件下的样品注入高效液相色谱-二极管阵列检测系统,以分析酚类物质的组成和含量。最佳提取条件为pH值为2的溶剂、70℃的温度和20分钟的提取时间。鉴定出了10种酚类物质:咖啡酸及其衍生物、绿原酸和阿魏酸、(+)-儿茶素、(-)-表儿茶素、芦丁和槲皮苷。这些酚类物质通常被报道具有重要的抗氧化、抗炎、抗衰老和抗癌活性,这使得这些残渣成为农业食品、化妆品或植物化学工业中生物活性化合物的优质来源。