Ibrahim Ayman M, Moss Matthew A, Gray Zane, Rojo Michelle D, Burke Caitlin M, Schwertfeger Kathryn L, Dos Santos Camila O, Machado Heather L
Department of Biochemistry and Molecular Biology, Tulane School of Medicine, New Orleans, LA, United States.
Tulane Cancer Center, Louisiana Cancer Research Consortium, New Orleans, LA, United States.
Front Oncol. 2020 Sep 24;10:569985. doi: 10.3389/fonc.2020.569985. eCollection 2020.
Myeloid cell heterogeneity remains poorly studied in breast cancer, and particularly in premalignancy. Here, we used single cell RNA sequencing to characterize macrophage diversity in mouse pre-invasive lesions as compared to lesions undergoing localized invasion. Several subpopulations of macrophages with transcriptionally distinct profiles were identified, two of which resembled macrophages in the steady state. While all subpopulations expressed tumor-promoting genes, many of the populations expressed pro-inflammatory genes, differing from reports in tumor-associated macrophages. Gene profiles of the myeloid cells were similar between early and late stages of premalignancy, although expansion of some subpopulations occurred. These results unravel macrophage heterogeneity in early progression and may provide insight into early intervention strategies that target macrophages.
在乳腺癌中，尤其是癌前病变阶段，髓样细胞的异质性仍未得到充分研究。在此，我们使用单细胞RNA测序来表征小鼠侵袭前病变与局部侵袭性病变中巨噬细胞的多样性。我们鉴定出了几个转录谱不同的巨噬细胞亚群，其中两个类似于稳态下的巨噬细胞。虽然所有亚群都表达促进肿瘤的基因，但许多亚群也表达促炎基因，这与肿瘤相关巨噬细胞的报道有所不同。癌前病变的早期和晚期阶段，髓样细胞的基因谱相似，不过一些亚群出现了扩增。这些结果揭示了早期进展过程中巨噬细胞的异质性，并可能为针对巨噬细胞的早期干预策略提供见解。
