Kiran Uday, Gokulan C G, Kuncha Santosh Kumar, Vedagiri Dhiviya, Chander Bingi Thrilok, Sekhar Aedula Vinaya, Dontamala Suchitra, Reddy Arakatla Lohith, Tallapaka Karthik Bharadwaj, Mishra Rakesh K, Harshan Krishnan Harinivas
CSIR-Centre for Cellular and Molecular Biology (CSIR-CCMB), Hyderabad, India.
Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, India.
Biol Methods Protoc. 2020 Aug 20;5(1):bpaa017. doi: 10.1093/biomethods/bpaa017. eCollection 2020.
Rigorous testing is the way forward to fight the coronavirus disease 2019 pandemic. Here we show that the currently used and most reliable reverse transcription-polymerase chain reaction-based severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) procedure can be further simplified to make it faster, safer, and economical by eliminating the RNA isolation step. The modified method is not only fast and convenient but also at par with the traditional method in terms of accuracy, and therefore can be used for mass screening. Our method takes about half the time and is cheaper by ∼40% compared to the currently used method. We also provide a variant of the new method that increases the efficiency of detection by ∼30% compared to the existing procedure. Taken together, we demonstrate a more effective and reliable method of SARS-CoV-2 detection.
严格检测是抗击2019冠状病毒病大流行的前进方向。在此我们表明，目前使用的、最可靠的基于逆转录-聚合酶链反应的严重急性呼吸综合征冠状病毒2（SARS-CoV-2）检测程序可以通过省去RNA分离步骤进一步简化，从而使其更快、更安全且更经济。改进后的方法不仅快速便捷，而且在准确性方面与传统方法相当，因此可用于大规模筛查。与目前使用的方法相比，我们的方法所需时间约为其一半，成本降低约40%。我们还提供了新方法的一个变体，与现有程序相比，其检测效率提高了约30%。综上所述，我们展示了一种更有效、更可靠的SARS-CoV-2检测方法。
