a Medical Faculty, University of Niš, Clinical center Niš-Clinic of urology, Niš, Serbia.
b Clinical-Hospital Center, Pristina, Gracanica, Serbia.
Can J Physiol Pharmacol. 2019 May;97(5):422-428. doi: 10.1139/cjpp-2018-0641. Epub 2019 Feb 7.
Acute kidney injury is a frequent disorder that can be mimicked by the application of different nephrotoxic agents, including carbon tetrachloride (CCl), where kidney injury marker-1 (KIM-1) has been recognized as a highly specific marker. Melatonin is one of the most powerful natural antioxidants and has numerous beneficial properties. We evaluated the nephroprotective potential of 2 melatonin treatment regimens (pre- and post-intoxication) in a CCl-induced acute kidney injury model based on the standard serum parameters, kidney tissue antioxidative capacity, KIM-1 levels, and kidney tissue morphological changes. The two treatment regimens were found to preserve kidney function, as judged from the evaluated standard serum parameters. Only when administered after the intoxication, melatonin preserved total kidney antioxidant capacity; pre-treatment melatonin only preserved reduced glutathione levels. An increase in tissue KIM-1 level was found to be prevented by both treatment regimens, which correlated with the morphological changes seen in the kidney tissues of animals treated with melatonin and CCl. The findings of our study are in agreement with the known actions of melatonin in relieving kidney tissue oxidative burden, but also contribute to the understanding of its action by preventing an increase in KIM-1.
急性肾损伤是一种常见的疾病，可能由不同的肾毒性物质引起，包括四氯化碳（CCl），其中肾损伤标志物-1（KIM-1）已被认为是一种高度特异性标志物。褪黑素是一种最强大的天然抗氧化剂之一，具有多种有益特性。我们基于标准血清参数、肾组织抗氧化能力、KIM-1 水平和肾组织形态学变化，评估了两种褪黑素治疗方案（中毒前和中毒后）在 CCl 诱导的急性肾损伤模型中的肾保护潜力。从评估的标准血清参数来看，这两种治疗方案都能保护肾功能。只有在中毒后给予褪黑素才能保持总肾抗氧化能力；预处理褪黑素仅能保持还原型谷胱甘肽水平。两种治疗方案均能预防组织 KIM-1 水平的升高，这与用褪黑素和 CCl 治疗的动物的肾组织形态变化相关。我们的研究结果与褪黑素缓解肾组织氧化应激的已知作用一致，但通过预防 KIM-1 的增加，也有助于了解其作用。
