Department of Animal Science, School of Agriculture and Biology, Shanghai Jiao Tong University, 800# Dongchuan Road, Shanghai, East, 200240, China.
Department of Animal Science, College of Animal Science, Zhejiang University, 866# Yuhangtang Road, Hangzhou, East, 310058, China.
Anim Genet. 2021 Feb;52(1):108-113. doi: 10.1111/age.13012. Epub 2020 Oct 18.
Intramuscular fat (IMF) is an important meat-quality trait of pigs, which influences pork's shearing force, hydraulics, tenderness and juicy flavor. However, to achieve a higher percentage of lean meat, pigs with lower backfat thickness (BF) are intensively selected for, which may lead to a reduction in pork quality. Therefore, the objective of this study was to locate loci that affect IMF without changing BF. A single-step GWAS was performed on 950 Duroc pigs genotyped by a 50K SNP chip in order to detect genomic variants relevant to IMF and BF. The significant SNPs detected were afterwards divided into a BF subset (seven SNPs), an IMF subset (11 SNPs) and a subset of both traits (12 SNPs), according to their P-value and LD. After SNP and QTL annotation, our results indicated that SSC1: 167938652, 166363826, 164829874 and 167171587 might be associated with IMF without changing BF. In the subset of both traits, we found that the combined effect of ALGA0006602 (SSC1: 159538854) and 12784636 (SSC1: 160773437) might improve the IMF without changing BF. Our gene annotation result showed that TLE3, ITGA11, SMAD6, PAQR5 and [RNF152 × MC4R ] genes might affect IMF independently of BF. We believe that the SNPs and genes identified in this study will be valuable for the future molecular breeding of IMF in Duroc pigs.
肌内脂肪（IMF）是猪的一个重要肉质特性，它影响猪肉的剪切力、水分保持力、嫩度和多汁性。然而，为了获得更高比例的瘦肉，人们对背膘厚度（BF）较低的猪进行了密集选择，这可能导致猪肉质量下降。因此，本研究的目的是定位不改变 BF 就能影响 IMF 的基因座。对 950 头杜洛克猪进行了单步 GWAS 分析，这些猪通过 50K SNP 芯片进行了基因型检测，以检测与 IMF 和 BF 相关的基因组变异。根据其 P 值和 LD，将检测到的显著 SNP 分为 BF 子集（7 个 SNP）、IMF 子集（11 个 SNP）和两个性状的子集（12 个 SNP）。在 SNP 和 QTL 注释之后，我们的结果表明，SSC1：167938652、166363826、164829874 和 167171587 可能与不改变 BF 的 IMF 有关。在两个性状的子集中，我们发现 ALGA0006602（SSC1：159538854）和 12784636（SSC1：160773437）的组合效应可能在不改变 BF 的情况下提高 IMF。我们的基因注释结果表明，TLE3、ITGA11、SMAD6、PAQR5 和 [RNF152×MC4R] 基因可能独立于 BF 影响 IMF。我们相信，本研究中鉴定的 SNP 和基因将对未来杜洛克猪 IMF 的分子育种具有重要价值。
