College of Animal Science and National Engineering Research Center for Breeding Swine Industry, South China Agricultural University, Guangzhou, 510642, China.
Guangdong Provincial Laboratory of Lingnan Modern Agricultural Science and Technology, Guangzhou, 510642, China.
Anim Genet. 2021 Aug;52(4):518-522. doi: 10.1111/age.13069. Epub 2021 May 31.
This study aimed at identifying genomic regions and genes associated with intramuscular fat content (IMF) in Duroc pigs using a weighted single-step GWAS. Data from 3912 pigs, of which 3770 animals were genotyped with GeneSeek Porcine 50K Bead chip, were used for the association analysis. We identified 19 genomic regions that each explained >1% of the additive genetic variance associated with IMF. Notably, a consistent QTL on SSC7 (117.42-117.92 Mb) was confirmed, explaining 3.70% of the additive genetic variance, and two genes, BDKRB2 and ATG2B, were highlighted as promising candidates for IMF. Two QTL (SSC7, 94.19-94.64 Mb; SSC14, 123.25-123.75 Mb), which harbored MED6 and MAP3K9 genes and TCF7L2 gene respectively, were newly identified as associated with IMF. In conclusion, we identified a consistent QTL and additional genomic regions and genes that contributed to the genetic variance of IMF using a large-scale sample size of genotyped pigs and genealogical information.
本研究旨在利用加权单步 GWAS 鉴定杜洛克猪肌肉内脂肪含量（IMF）相关的基因组区域和基因。使用了来自 3912 头猪的数据，其中 3770 头动物用 GeneSeek Porcine 50K Bead 芯片进行了基因分型，用于关联分析。我们鉴定了 19 个基因组区域，每个区域解释了与 IMF 相关的加性遗传变异的>1%。值得注意的是，在 SSC7（117.42-117.92 Mb）上确认了一个一致的 QTL，解释了 3.70%的加性遗传变异，并且 BDKRB2 和 ATG2B 两个基因被突出为 IMF 的有希望的候选基因。两个 QTL（SSC7，94.19-94.64 Mb；SSC14，123.25-123.75 Mb）分别携带 MED6 和 MAP3K9 基因和 TCF7L2 基因，被新鉴定为与 IMF 相关。总之，我们使用大量基因分型猪和系谱信息的样本大小鉴定了一个一致的 QTL 和其他与 IMF 遗传变异相关的基因组区域和基因。
