Zhang Ning, Sun Nianrong, Deng Chunhui
Department of Chemistry, Fudan University, Shanghai, 200433, China.
Department of Gastroenterology, Zhongshan Hospital of Fudan University, Shanghai, 200032, China.
Talanta. 2021 Jan 1;221:121571. doi: 10.1016/j.talanta.2020.121571. Epub 2020 Sep 1.
There are accumulating evidence that proteins carried by exosomes in urine are most possibly used as biomarkers or therapeutic carriers for certain diseases. The isolation of exosomes is therefore highly desirable for aiding the downstream protein analysis. Particularly, urine is a dynamic biological fluid changing within a short time, resulting in that the separation of urinary exosome requires more efficient technology. Here, a new biocompatible material (denoted as FeO@TiO-CD63 aptamer) is designed and synthesized for rapid exosome isolation from human urine, depending on the double interactions of TiO with phosphate groups as well as aptamers with specific exosome proteins. Moreover, within 10 min, 92.6% exosomes with intact structure are captured from urine by FeO@TiO-CD63 aptamers, from which 999 proteins are detected through LC-MS/MS.
越来越多的证据表明，尿液中外泌体携带的蛋白质很可能用作某些疾病的生物标志物或治疗载体。因此，非常需要分离外泌体以辅助下游蛋白质分析。特别是，尿液是一种在短时间内变化的动态生物流体，这导致尿外泌体的分离需要更高效的技术。在此，设计并合成了一种新型生物相容性材料（表示为FeO@TiO-CD63适配体），用于从人尿中快速分离外泌体，这依赖于TiO与磷酸基团以及适配体与特定外泌体蛋白的双重相互作用。此外，在10分钟内，FeO@TiO-CD63适配体从尿液中捕获了92.6%结构完整的外泌体，通过LC-MS/MS从中检测到999种蛋白质。
