Miura K, Yukimura T, Yamada H, Nakamura N, Ito K, Ikemoto F, Ogita S, Yamamoto K
Department of Pharmacology, Osaka City University Medical School, Japan.
Clin Exp Hypertens A. 1987;9(8-9):1455-65. doi: 10.3109/10641968709158995.
A solid phase sandwich assay for human renin was used to determine the total renin concentration in plasma throughout the course of a normal pregnancy. These values were compared with the enzymatic activity of renin, with and without trypsin treatment. Plasma concentration of immunoreactive renin rapidly increased four fold from 214 +/- 33 pg/ml (in non-pregnant subjects) within a few weeks after conception and remained high up to the third trimester. Total renin concentration, estimated by trypsin-activated enzyme activity showed similar changes, both qualitatively and quantitatively. A positive correlation (r = 0.63, p less than 0.001) between immunoreactive renin concentration and its enzymatic activity was noted whereas the active renin concentration did not positively correlate with the immunoreactive renin concentration. These data indicate that solid phase sandwich assay for human renin should be a pertinent tool to elucidate the role of the renin-angiotensin system in pregnancy.
采用人肾素固相夹心测定法测定正常妊娠全过程血浆中的总肾素浓度。将这些值与肾素的酶活性进行比较，包括有无胰蛋白酶处理的情况。免疫反应性肾素的血浆浓度在受孕后几周内从214±33 pg/ml（非妊娠受试者）迅速增加了四倍，并在妊娠晚期一直保持在较高水平。通过胰蛋白酶激活的酶活性估计的总肾素浓度在定性和定量方面都显示出类似的变化。免疫反应性肾素浓度与其酶活性之间呈正相关（r = 0.63，p < 0.001），而活性肾素浓度与免疫反应性肾素浓度无正相关。这些数据表明，人肾素固相夹心测定法应是阐明肾素 - 血管紧张素系统在妊娠中作用的相关工具。
