Department of Osteoporosis Care and Control, The First People's Hospital of Xiaoshan District, Hangzhou, Zhejiang 311200, China.
Institute of Orthopaedics and Traumatology of Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, China.
Biomed Res Int. 2020 Oct 1;2020:4951251. doi: 10.1155/2020/4951251. eCollection 2020.
Circular RNAs (circRNAs) have been found to contribute to the regulation of many diseases and are abundantly expressed in various organisms. The present study is aimed at systematically characterizing the circRNA expression profiles in patients with senile osteoporotic vertebral compression fracture (OVCF) and predicting the potential functions of the regulatory networks correlated with these differentially expressed circRNAs.
The circRNA expression profile in patients with senile OVCF was explored by using RNA sequencing. The prediction of the enriched signaling pathways and circRNA-miRNA networks was conducted by bioinformatics analysis. Real-time quantitative PCR was used to validate the selected differentially expressed circRNAs from 20 patients with senile OVCF relative to 20 matched healthy controls.
A total of 884 differentially expressed circRNAs were identified, of which 554 were upregulated and 330 were downregulated. The top 15 signaling pathways associated with these differentially expressed circRNAs were predicted. The result of qRT-PCR of the selected circRNAs was consistent with RNA sequencing.
CircRNAs are differentially expressed in patients with senile OVCF, which might contribute to the pathophysiological mechanism of senile osteoporosis.
环状 RNA（circRNAs）已被发现有助于多种疾病的调控，并且在各种生物体中大量表达。本研究旨在系统地表征老年骨质疏松性椎体压缩性骨折（OVCF）患者的 circRNA 表达谱，并预测与这些差异表达 circRNAs 相关的调控网络的潜在功能。
采用 RNA 测序技术探讨老年 OVCF 患者的 circRNA 表达谱。通过生物信息学分析预测富集的信号通路和 circRNA-miRNA 网络。采用实时定量 PCR 技术对 20 例老年 OVCF 患者与 20 例匹配的健康对照者的部分差异表达 circRNAs 进行验证。
共鉴定出 884 个差异表达的 circRNAs，其中 554 个上调，330 个下调。预测了与这些差异表达 circRNAs 相关的前 15 个信号通路。所选 circRNAs 的 qRT-PCR 结果与 RNA 测序一致。
老年 OVCF 患者的 circRNAs 表达存在差异，这可能有助于老年骨质疏松症的病理生理机制。
