Gheidari Davood, Mehrdad Morteza, Maleki Saloomeh, Hosseini Samanesadat
Department of Chemistry, Faculty of Science, University of Guilan, Iran.
Department of Chemistry, Faculty of Science, University of Shahrood, Iran.
Heliyon. 2020 Oct 8;6(10):e05058. doi: 10.1016/j.heliyon.2020.e05058. eCollection 2020 Oct.
The nanoparticles of Cobalt ferrite are synthesized using polyethylene glycol as a solvent by the solvothermal method in a surfactant-free condition. Nanoparticles that were synthesized were determined by using various techniques such as Diffuse Reflection Spectroscopy (DRS), X-Ray Diffraction (XRD), Scanning Electron Microscope (SEM), and Energy Dispersive X-ray spectroscopy (EDAX). The Scanning electron microscope confirmed the range of spherical nanoparticles in the size of 20-33 nm.An excellent match was observed between the calculated particles size in the X-ray diffraction and electron microscopes results. Furthermore, their antimicrobial efficacy was determined by MIC, MBC, IC50 and disc diffusion method on Gram-negative ( and ) and Gram-positive () bacteria. The results indicated an acceptable bacteriostatic and bactericidal effects of this nanoparticles. Additionally, it was seen that by the increase in the concentration of nanoparticles, their antimicrobial property would increase.
In recent years, antibacterial materials have found a special place to avoid the overuse of antibiotics. In this study, the antibacterial effects of CoFeO nanoparticles on were investigated due to their importance as human pathogens in nosocomial infection.
In this study, the antibacterial effects of CoFeO nanoparticles such as MIC, MBC, IC50, and disc diffusion method were examined.
According to the results, CoFeO nanoparticles exhibited potent antibacterial activity against the bacteria that were examined, especially . The MBC (Minimum Bactericidal Concentration) of CoFeO nanoparticle on , , , was between 0.12-0.48 mg/ml and MIC (Minimum Inhibition Concentration) on these bacteria detected between 0.06-0.24 mg/ml. The least IC50 determined for with a concentration of 0.061 mg/ml. and identified as the most resistant and sensitive bacteria in the disc diffusion method, respectively.
采用聚乙二醇作为溶剂，通过溶剂热法在无表面活性剂条件下合成了钴铁氧体纳米颗粒。通过漫反射光谱（DRS）、X射线衍射（XRD）、扫描电子显微镜（SEM）和能量色散X射线光谱（EDAX）等多种技术对合成的纳米颗粒进行了表征。扫描电子显微镜证实了球形纳米颗粒的尺寸范围为20 - 33纳米。在X射线衍射计算的颗粒尺寸与电子显微镜结果之间观察到了极好的匹配。此外，通过最小抑菌浓度（MIC）、最小杀菌浓度（MBC）、半数抑制浓度（IC50）和纸片扩散法测定了它们对革兰氏阴性菌（和）和革兰氏阳性菌（）的抗菌效果。结果表明该纳米颗粒具有可接受的抑菌和杀菌作用。此外，还发现随着纳米颗粒浓度的增加，其抗菌性能会增强。
近年来，抗菌材料在避免抗生素过度使用方面占据了特殊地位。在本研究中，鉴于钴铁氧体纳米颗粒作为医院感染中人类病原体的重要性，对其抗菌效果进行了研究。
在本研究中，通过最小抑菌浓度（MIC）、最小杀菌浓度（MBC）、半数抑制浓度（IC50）和纸片扩散法等方法研究了钴铁氧体纳米颗粒的抗菌效果。
根据结果，钴铁氧体纳米颗粒对所检测的细菌表现出强大的抗菌活性，尤其是。钴铁氧体纳米颗粒对、、、的最小杀菌浓度（MBC）在0.12 - 0.48毫克/毫升之间，对这些细菌的最小抑菌浓度（MIC）在0.06 - 0.24毫克/毫升之间。对的半数抑制浓度（IC50）最低，为0.061毫克/毫升。在纸片扩散法中，和分别被确定为最耐药和最敏感的细菌。
