School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, China.
Guangdong Xinbaotang Biological Technology Co. Ltd., Guangzhou 529000, China.
J Pharm Biomed Anal. 2021 Jan 5;192:113683. doi: 10.1016/j.jpba.2020.113683. Epub 2020 Oct 14.
Citrus reticulata cv. Chachiensis (CRC) has been widely used in many Chinese medicine prescriptions and developed as various health products. Due to its extensive biological activities, increased interest has focused on CRC as a potential source for the treatment of various diseases. However, there is no systematic and sufficient research on optimization and characterization of ultrasound extraction of antioxidant compounds from CRC up to now. In the present work, three parameters including ultrasonic amplitude (45, 55, 70, 85, 95 %), ultrasonic time (26, 40, 60, 80, 94 min) and ethanol concentration (16, 30, 50, 70, 84 %, v/v) were estimated using central-composite design. Determined optimum conditions, considering four responses (TPC, TFC, DPPH and ABTS) at the same time, were 72 % amplitude, 80 min and 70 % ethanol concentration. The maximum yields were TPC values (145.73 mg GAE/g), TFC values (135.21 mg QE/g), DPPH and ABTS scavenging capacity (87.88 and 76.18 mg TE/g, respectively). 55 phytochemicals were identified in the CRC extract using the UPLC-QTOF-MS/MS, 34 of which were flavonoids, which provided important support for various potential applications of CRC.
枳椇（Citrus reticulata cv. Chachiensis，CRC）在许多中药方剂中被广泛应用，并被开发成各种保健品。由于其广泛的生物活性，CRC 作为治疗各种疾病的潜在来源引起了越来越多的关注。然而，目前还没有关于超声提取 CRC 中抗氧化化合物的优化和表征的系统和充分的研究。在本工作中，采用中心组合设计法对超声幅度（45、55、70、85、95%）、超声时间（26、40、60、80、94 min）和乙醇浓度（16、30、50、70、84%，v/v）三个参数进行了评估。同时考虑四个响应（TPC、TFC、DPPH 和 ABTS），确定的最佳条件为 72%的振幅、80 分钟和 70%的乙醇浓度。最大产率分别为 TPC 值（145.73mgGAE/g）、TFC 值（135.21mgQE/g）、DPPH 和 ABTS 清除能力（87.88 和 76.18mgTE/g）。采用 UPLC-QTOF-MS/MS 在 CRC 提取物中鉴定了 55 种植物化学物质，其中 34 种为类黄酮，为 CRC 的各种潜在应用提供了重要支持。
