Key Laboratory of Molecular Biophysics of Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, Hubei, 430074, China.
Department of Cerebrovascular Diseases, The Second Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, 450014, China.
Sci Data. 2020 Oct 21;7(1):361. doi: 10.1038/s41597-020-00709-6.
Shift work is known to be associated with an increased risk of neurological and psychiatric diseases, but how it contributes to the development of these diseases remains unclear. Chronic jet lag (CJL) induced by shifting light-dark cycles repeatedly is a commonly used protocol to mimic the environmental light/dark changes encountered by shift workers. Here we subjected wildtype mice to CJL and performed positron emission tomography imaging of glucose metabolism to monitor brain activities. We also conducted RNA sequencing using prefrontal cortex and nucleus accumbens tissues from these animals, which are brain regions strongly implicated in the pathology of various neurological and psychiatric conditions. Our results reveal the alterations of brain activities and systematic reprogramming of gene expression in brain tissues under CJL, building hypothesis for how CJL increases the susceptibility to neurological and psychiatric diseases.
轮班工作已知与神经和精神疾病的风险增加有关，但它如何导致这些疾病的发展尚不清楚。反复的光暗周期变化引起的慢性时差（CJL）是模拟轮班工人遇到的环境光/暗变化的常用方案。在这里，我们让野生型小鼠接受 CJL，并进行葡萄糖代谢的正电子发射断层扫描成像，以监测大脑活动。我们还使用这些动物的前额叶皮层和伏隔核组织进行了 RNA 测序，这些区域强烈参与各种神经和精神疾病的病理过程。我们的研究结果揭示了 CJL 下大脑活动的改变和脑组织中基因表达的系统性重编程，为 CJL 如何增加患神经和精神疾病的易感性提供了假设。
