College of Food and Biology Engineering, Xuzhou University of Technology, Xuzhou, Jiangsu, China.
Xuzhou Technology Limited Company of United Gene, Xuzhou, Jiangsu, China.
PLoS One. 2020 Oct 22;15(10):e0241078. doi: 10.1371/journal.pone.0241078. eCollection 2020.
The present study aimed to determine whether the polymorphisms at rs2241766 and rs1501299 on the ADIPOQ gene were related to the susceptibility of type 2 diabetes mellitus (T2DM).
Eight databases, PubMed, GWAS, Embase, Lochrane, Ebsco, CNKI (Chinese National Knowledge Infrastructure), VIP (Viper Database) and ChinaInfo were searched, and a meta-analysis of susceptibility was conducted between SNP45, SNP276 polymorphisms and T2DM. Furthermore, HWE test was conducted to assess the genetic balance of the study, evaluate the quality of Newcastle-Ottawa quality assessment scale (NOS), and establishing allelic, dominant, recessive, heterozygous, and homozygous gene models.
This meta-analysis included 53 articles, encompassing 9285 cases with rs2241766 and 14156 controls and 7747 cases with rs1501299 and 10607 controls. For the rs2241766 locus, a significant correlation was found in the three models by the subgroup analysis. Western Asians: dominant gene model (TT + TG vs. GG, P = 0.01); heterozygous gene model (TG vs. GG, P = 0.02); homozygous gene model (TT vs. GG, P = 0.01). South Asians: dominant gene model (TT + TG vs. GG, P = 0.004); heterozygous gene model (TG vs. GG, P = 0.009); homozygous gene model (TT vs. GG, P = 0.005). However, no statistically significant correlation was established among the five genetic models for rs1501299 locus.
The findings of the present study indicated that the T allele of rs2241766 polymorphism is the susceptibility locus of T2DM in the West Asian population, but has a protective effect in the South Asian population, albeit further studies are needed in other populations. Also, no association was found between the ADIPOQ rs1501299 polymorphism and T2DM.
本研究旨在探讨 ADIPOQ 基因 rs2241766 和 rs1501299 多态性与 2 型糖尿病（T2DM）易感性的关系。
检索PubMed、GWAS、Embase、Lochrane、Ebsco、CNKI（中国国家知识基础设施）、VIP（Viper 数据库）和 ChinaInfo 共 8 个数据库，对 SNP45、SNP276 多态性与 T2DM 的易感性进行荟萃分析。此外，还进行了 HWE 检验以评估研究的遗传平衡，评价 Newcastle-Ottawa 质量评估量表（NOS）的质量，并建立等位基因、显性、隐性、杂合子和纯合子基因模型。
本荟萃分析共纳入 53 篇文献，包括 rs2241766 位点的 9285 例病例和 14156 例对照，以及 rs1501299 位点的 7747 例病例和 10607 例对照。亚组分析显示，rs2241766 位点在三种模型中均存在显著相关性。在西方人群中：显性基因模型（TT+TG 与 GG，P=0.01）；杂合子基因模型（TG 与 GG，P=0.02）；纯合子基因模型（TT 与 GG，P=0.01）。在南亚人群中：显性基因模型（TT+TG 与 GG，P=0.004）；杂合子基因模型（TG 与 GG，P=0.009）；纯合子基因模型（TT 与 GG，P=0.005）。然而，rs1501299 位点的五种遗传模型之间没有建立统计学上的显著相关性。
本研究结果表明，rs2241766 多态性的 T 等位基因是西方人群中 T2DM 的易感位点，但在南亚人群中具有保护作用，但需要在其他人群中进一步研究。此外，ADIPOQ rs1501299 多态性与 T2DM 无关。
