Tu Dom-Gene, Chyau Charng-Cherng, Chen Shih-Ying, Chu Heuy-Ling, Wang Shu-Chen, Duh Pin-Der
Department of Nuclear Medicine, Ditmanson Medical Foundation, Chia-Yi Christian Hospital, Chiayi 600, Taiwan.
Department of Biomedical Sciences, National Chung Cheng University, Minhsiung, Chia-yi 62102, Taiwan.
Foods. 2020 Oct 21;9(10):1514. doi: 10.3390/foods9101514.
The antiproliferative effect and mediation of apoptosis in human hepatoma HepG2 cells induced by djulis husk and its bioactive compounds was investigated. The ethanolic extracts of djulis husk (EEDH) at 50, 250, and 500 µg/mL induced remarkable cytotoxicity on HepG2 cells. By flow cytometry analysis, EEDH slowed down the cell cycle at the Sub-G0 phase after 24 h of incubation. Moreover, all EEDH treatment induced an apoptotic response in HepG2 cells. EEDH-induced apoptosis was associated with the attenuation of mitochondrial transmembrane potentials (ΔΨ), an increase in Bax/Bcl-2 ratio, activation of caspase-3, and poly(ADP-ribose)polymerase (PARP) cleavage, as well as an increase in reactive oxygen species (ROS) generation. According to the HPLC-DAD and HPLC-MS/MS analysis, quercetin and kaempferol derivatives and another sixteen compounds were present in EEDH. Quercetin and kaempferol at 25-150 μM showed antiproliferative action and induced apoptosis on HepG2 cells, which may in part account for the anticancer activity of EEDH. Overall, EEDH may be a potent chemopreventive agent due to apoptosis in HepG2 cells.
研究了薏仁壳及其生物活性化合物对人肝癌HepG2细胞的抗增殖作用和凋亡介导作用。薏仁壳乙醇提取物（EEDH）在50、250和500μg/mL浓度下对HepG2细胞具有显著的细胞毒性。通过流式细胞术分析，孵育24小时后，EEDH使细胞周期在Sub-G0期减缓。此外，所有EEDH处理均诱导HepG2细胞发生凋亡反应。EEDH诱导的凋亡与线粒体跨膜电位（ΔΨ）的减弱、Bax/Bcl-2比值的增加、caspase-3的激活、聚（ADP-核糖）聚合酶（PARP）的裂解以及活性氧（ROS）生成的增加有关。根据HPLC-DAD和HPLC-MS/MS分析，EEDH中存在槲皮素和山奈酚衍生物以及另外16种化合物。25-150μM的槲皮素和山奈酚对HepG2细胞具有抗增殖作用并诱导凋亡，这可能部分解释了EEDH的抗癌活性。总体而言，由于EEDH可诱导HepG2细胞凋亡，它可能是一种有效的化学预防剂。
