School of Health Sciences, Universidade Positivo, Rua Prof. Pedro Viriato Parigot de Souza, 5300-Campo Comprido, Curitiba, PR, 80060-000, Brazil.
Department of Pediatric Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo, Avenida do Café, Ribeirao Preto, SP, 14040-904, Brazil.
Clin Oral Investig. 2021 May;25(5):3249-3255. doi: 10.1007/s00784-020-03655-0. Epub 2020 Oct 25.
The aim of this study was investigate the cranium dimensions of adult female rats, who suffered estrogen deficiency during the prepubertal stage, to assess the impact of estrogen deficiency on craniofacial morphology.
Twenty-two female Wistar rats were divided into ovariectomy (OVX) (n = 11) and sham-operated control (n = 11) groups. Bilateral ovariectomy were performed in both groups at 21 days old (prepubertal stage), and rats were euthanized at an age of 63 days (post-pubertal stage). Micro-CT scans were performed with rat skulls, and the cranium morphometric landmark measurements were taken in the dorsal, lateral, and ventral view positions. Differences in measurements between the OVX and sham control groups were assessed using t test with an established alpha error of 5%.
The measures of the rats' skull showed that the inter-zygomatic arch width and anterior cranial base length were significantly larger in OVX group (p = 0.020 and p = 0.050, respectively), whereas the length of parietal bone was significantly higher in the sham group (p = 0.026). For the remaining measurements no significant differences between groups were detected (p > 0.05).
This study provides evidence that ovariectomized rats had alterations in cranial bone dimensions, demonstrating that estrogens during puberty are important for skull morphology.
To understand the role of estrogen on the postnatal cranium development will impact the clinical diagnose and therapy during childhood and adolescence.
本研究旨在探讨青春期前雌激素缺乏雌性大鼠的颅部尺寸，以评估雌激素缺乏对颅面形态的影响。
将 22 只雌性 Wistar 大鼠分为卵巢切除术（OVX）组（n = 11）和假手术对照组（n = 11）。两组均在 21 天大（青春期前）时行双侧卵巢切除术，63 天大（青春期后）时处死大鼠。对大鼠颅骨进行 micro-CT 扫描，并在背侧、外侧和腹侧位置进行颅形态测量标志点测量。使用 t 检验评估 OVX 组和假手术对照组之间测量值的差异，并设定 5%的置信区间。
大鼠颅骨的测量值显示，OVX 组的眶间弓宽度和颅前底长度显著增大（p = 0.020 和 p = 0.050），而假手术组的顶骨长度显著增高（p = 0.026）。其余测量值两组间无显著差异（p > 0.05）。
本研究提供了证据表明，去卵巢大鼠的颅骨尺寸发生了改变，表明青春期雌激素对颅骨形态的重要性。
了解雌激素对出生后颅部发育的作用将影响儿童和青少年时期的临床诊断和治疗。
