Department of Biology, MIT BioMicro Center, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
J Biomol Tech. 2020 Dec;31(4):151-156. doi: 10.7171/jbt.20-3104-004.
Advances in next-generation sequencing technologies have allowed RNA sequencing to become an increasingly time efficient, cost-effective, and accessible tool for genomic research. We present here an automated and miniaturized workflow for RNA library preparation that minimizes reagent usage and processing time required per sample to generate Illumina compatible libraries for sequencing. The reduced-volume libraries show similar behavior to full-scale libraries with comparable numbers of genes detected and reproducible clustering of samples.
下一代测序技术的进步使得 RNA 测序成为基因组研究中一种越来越高效、经济、便捷的工具。我们在此展示了一种自动化和微型化的 RNA 文库制备工作流程，该流程最大限度地减少了每个样本所需的试剂用量和处理时间，可生成适用于 Illumina 测序的文库。与全规模文库相比，减少体积的文库具有相似的行为，可检测到可比数量的基因，并且样本聚类具有可重复性。
