Shuai Cijun, Guo Wang, Gao Chengde, Yang Youwen, Wu Ping, Feng Pei
State Key Laboratory of High Performance Complex Manufacturing, College of Mechanical and Electrical Engineering, Central South University, Changsha, China.
Jiangxi University of Science and Technology, Ganzhou, China.
Int J Bioprint. 2017 Nov 1;4(1):120. doi: 10.18063/IJB.v4i1.120. eCollection 2018.
Bone repair failure caused by implant-related infections is a common and troublesome problem. In this study, an antibacterial scaffold was developed via selective laser sintering with incorporating nano magnesium oxide (nMgO) to poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV). The results indicated the scaffold exerted high antibacterial activity. The antibacterial mechanism was that nMgO could cause oxidative damage and mechanical damage to bacteria through the production of reactive oxygen species (ROS) and direct contact action, respectively, which resulted in the damage of their structures and functions. Besides, nMgO significantly increased the compressive properties of the scaffold including strength and modulus, due to its excellent mechanical properties and uniform dispersion in the PHBV matrix. Moreover, the degradation tests indicated nMgO neutralized the acid degradation products of PHBV and benefited the degradation of the scaffold. The cell culture demonstrated that nMgO promoted the cellular adhesion and proliferation, as well as osteogenic differentiation. The present work may open the door to exploring nMgO as a promising antibacterial material for tissue engineering.
由植入物相关感染引起的骨修复失败是一个常见且棘手的问题。在本研究中，通过选择性激光烧结，将纳米氧化镁（nMgO）掺入聚（3-羟基丁酸酯-co-3-羟基戊酸酯）（PHBV）中制备了一种抗菌支架。结果表明该支架具有高抗菌活性。抗菌机制是nMgO可分别通过产生活性氧（ROS）和直接接触作用对细菌造成氧化损伤和机械损伤，从而导致细菌结构和功能的破坏。此外，由于nMgO具有优异的力学性能且在PHBV基体中均匀分散，它显著提高了支架的压缩性能，包括强度和模量。而且，降解试验表明nMgO中和了PHBV的酸性降解产物并有利于支架的降解。细胞培养显示nMgO促进了细胞的黏附、增殖以及成骨分化。目前的工作可能为探索nMgO作为一种有前景的组织工程抗菌材料打开大门。
