Maldonado-Rodríguez Erika, Hernández-Barrales Marisa, Reyes-López Adrián, Godina-González Susana, Gallegos-Flores Perla I, Esparza-Ibarra Edgar L, González-Curiel Irma E, Aguayo-Rojas Jesús, López-Saucedo Adrián, Mendoza-Almanza Gretel, Ayala-Luján Jorge L
Master in Science and Chemical Technology, Autonomous University of Zacatecas, Zacatecas 98160, Mexico.
Academic Unit of Chemical Sciences, Autonomous University of Zacatecas, Zacatecas 98160, Mexico.
Curr Issues Mol Biol. 2022 Aug 13;44(8):3648-3665. doi: 10.3390/cimb44080250.
Breast cancer is the leading cause of cancer death among women worldwide. Multiple extrinsic and intrinsic factors are associated with this disease's development. Various research groups worldwide have reported the presence of human papillomavirus (HPV) DNA in samples of malignant breast tumors. Although its role in mammary carcinogenesis is not fully understood, it is known that the HPV genome, once inserted into host cells, has oncogenic capabilities. The present study aimed to detect the presence of HPV DNA in 116 breast tissue biopsies and classify them according to their histology. It was found that 50.9% of the breast biopsies analyzed were malignant neoplasms, of which 74.6% were histologically classified as infiltrating ductal carcinoma. In biopsies with non-malignant breast disease, fibroadenoma was the most common benign neoplasm (39.1%). Detection of HPV DNA was performed through nested PCR using the external primer MY09/11 and the internal primer GP5+/6+. A hybridization assay genotyped HPV. HPV DNA was identified in 20.3% (12/59) of malignant neoplasms and 35% non-malignant breast disease (16/46). It was also detected in 27.3% (3/11) of breast tissue biopsies without alteration. However, there are no statistically significant differences between these groups and the existence of HPV DNA ( = 0.2521). Its presence was more frequent in non-malignant alterations than in malignant neoplasias. The most frequent genotypes in the HPV-positive samples were low-risk (LR) HPV-42 followed by high-risk (HR) HPV-31.
乳腺癌是全球女性癌症死亡的主要原因。多种外在和内在因素与这种疾病的发展相关。世界各地的多个研究小组报告了在恶性乳腺肿瘤样本中存在人乳头瘤病毒（HPV）DNA。尽管其在乳腺癌发生中的作用尚未完全了解，但已知HPV基因组一旦插入宿主细胞，就具有致癌能力。本研究旨在检测116份乳腺组织活检样本中HPV DNA的存在，并根据其组织学进行分类。结果发现，所分析的乳腺活检样本中有50.9%为恶性肿瘤，其中74.6%在组织学上被分类为浸润性导管癌。在患有非恶性乳腺疾病的活检样本中，纤维腺瘤是最常见的良性肿瘤（39.1%）。通过使用外部引物MY09/11和内部引物GP5+/6+的巢式PCR检测HPV DNA。采用杂交试验对HPV进行基因分型。在20.3%（12/59）的恶性肿瘤和35%的非恶性乳腺疾病（16/46）中鉴定出HPV DNA。在无病变的乳腺组织活检样本中也检测到27.3%（3/11）。然而，这些组之间HPV DNA的存在没有统计学上的显著差异（P = 0.2521）。其在非恶性病变中的存在比在恶性肿瘤中更频繁。HPV阳性样本中最常见的基因型是低风险（LR）HPV-42，其次是高风险（HR）HPV-31。
