Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
Tick-Pathogen Transmission Unit, Laboratory of Bacteriology, National Institute of Allergy and Infectious Diseases, Hamilton, USA.
Sci Rep. 2020 Oct 26;10(1):18296. doi: 10.1038/s41598-020-75341-w.
To further obtain insights into the Rhipicephalus microplus transcriptome, we used RNA-seq to carry out a study of expression in (i) embryos; (ii) ovaries from partially and fully engorged females; (iii) salivary glands from partially engorged females; (iv) fat body from partially and fully engorged females; and (v) digestive cells from partially, and (vi) fully engorged females. We obtained > 500 million Illumina reads which were assembled de novo, producing > 190,000 contigs, identifying 18,857 coding sequences (CDS). Reads from each library were mapped back into the assembled transcriptome giving a view of gene expression in different tissues. Transcriptomic expression and pathway analysis showed that several genes related in blood digestion and host-parasite interaction were overexpressed in digestive cells compared with other tissues. Furthermore, essential genes for the cell development and embryogenesis were overexpressed in ovaries. Taken altogether, these data offer novel insights into the physiology of production and role of saliva, blood digestion, energy metabolism, and development with submission of 10,932 novel tissue/cell specific CDS to the NCBI database for this important tick species.
为了进一步深入了解璃眼蜱转录组，我们使用 RNA-seq 对（i）胚胎；（ii）部分和完全饱血的雌性卵巢；（iii）部分饱血的雌性唾液腺；（iv）部分和完全饱血的雌性血体；以及（v）部分和（vi）完全饱血的雌性消化细胞进行了表达研究。我们获得了超过 5 亿个 Illumina 读段，通过从头组装，生成了超过 190000 个 contigs，鉴定出了 18857 个编码序列（CDS）。来自每个文库的读段被映射回组装的转录组，从而可以观察到不同组织中的基因表达情况。转录组表达和通路分析表明，与其他组织相比，消化细胞中与血液消化和宿主-寄生虫相互作用相关的几个基因表达上调。此外，对细胞发育和胚胎发生至关重要的基因在卵巢中表达上调。总的来说，这些数据为唾液、血液消化、能量代谢和发育的生理学提供了新的见解，并向 NCBI 数据库提交了 10932 个新的组织/细胞特异性 CDS，为这种重要的蜱种提供了新的见解。
