Knibbe C A, Koster V S, Deneer V H, Stuurman R M, Kuks P F, Lange R
Department of Clinical Pharmacy, St. Anonius Hospital, Nieuwegein, The Netherlands.
J Chromatogr B Biomed Sci Appl. 1998 Mar 20;706(2):305-10. doi: 10.1016/s0378-4347(97)00571-9.
In order to determine propofol in rat whole-blood samples of 50 microl, we developed a rapid, simple and reliable method which is characterized by precipitation of blood elements with acetonitrile and submission of the supernatant to HPLC analysis with fluorescence detection. The method described is linear from 0.4 to 40 mg/l and the relative standard deviations in this concentration range are less than 10%. The limit of quantification proved to be 0.4 mg/l. Blood constituents do not interfere with the assay.
为测定50微升大鼠全血样本中的丙泊酚，我们开发了一种快速、简便且可靠的方法，其特点是用乙腈沉淀血液成分，然后将上清液进行具有荧光检测的高效液相色谱分析。所述方法在0.4至40毫克/升范围内呈线性，此浓度范围内的相对标准偏差小于10%。定量限为0.4毫克/升。血液成分不干扰该测定。
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