Institute of Experimental Medicine, Faculty of Medicine, P. J. Šafárik University, Košice, Slovak Republic.
Acta Biochim Pol. 2020 Oct 27;67(4):447-451. doi: 10.18388/abp.2020_5404.
The aim of this study was to investigate the possibilities of modification of chronic disease risk factors with probiotic strain Lactobacillus plantarum LS/07 and prebiotic inulin in rats with western high fat diet. The Sprague-Dawley rats were divided into four groups: control group (CG group), group with high fat diet (HFD group), group receiving high fat diet in combination with Lactobacillus plantarum LS/07 (HFD+PRO group), and group receiving high fat diet in combination with oligofructose enriched inulin (HFD+PRE group). The activity of β-glucuronidase, lipid parameters, bile acids, oxLDL, short chain fatty acids, and counts of coliforms and lactobacilli were determined. High fat diet as a key risk factor of chronic diseases had adverse effect on expression of metabolic and biochemical parameters. Dietary intake of Lactobacillus plantarum LS/07 (HFD+PRO group) and inulin (HFD+PRE group) suppressed weight gain of rats. In HFD+PRO group, the level of total cholesterol (P<0.001), LDL-CH (P<0.05), oxLDL (P<0.001), total bile acids (P<0.001) were statistically significantly decreased, while the production of short chain fatty acids was enhanced. Changes in the selected parameters exhibited a similar tendency also in the HFD+PRE group. Activity of β-glucuronidase was statistically significantly decreased (P<0.001) in the HFD+PRE group. Lactobacillus plantarum LS/07 and inulin caused a statistically significant increase in the count of lactobacilli (P<0.001) and a decrease in the number of coliforms (P<0.001). These results indicate Lactobacillus plantarum LS/07 and inulin could be used in diet for human and animals as an important nutritional supplement or in medicinal products.
本研究旨在探讨利用益生菌植物乳杆菌 LS/07 和益生元菊粉对西方高脂肪饮食大鼠的慢性疾病风险因素进行修饰的可能性。将 Sprague-Dawley 大鼠分为四组：对照组（CG 组）、高脂肪饮食组（HFD 组）、接受植物乳杆菌 LS/07 联合高脂肪饮食的组（HFD+PRO 组）和接受富含低聚果糖菊粉的高脂肪饮食的组（HFD+PRE 组）。测定β-葡萄糖醛酸酶活性、脂质参数、胆汁酸、氧化型低密度脂蛋白（oxLDL）、短链脂肪酸以及大肠埃希菌和乳酸菌的计数。高脂肪饮食作为慢性疾病的关键风险因素，对代谢和生化参数的表达有不良影响。植物乳杆菌 LS/07（HFD+PRO 组）和菊粉（HFD+PRE 组）的饮食摄入抑制了大鼠的体重增加。在 HFD+PRO 组中，总胆固醇（P<0.001）、LDL-CH（P<0.05）、oxLDL（P<0.001）和总胆汁酸（P<0.001）水平均有统计学显著降低，而短链脂肪酸的产生增强。所选参数的变化在 HFD+PRE 组也表现出相似的趋势。β-葡萄糖醛酸酶活性在 HFD+PRE 组也有统计学显著降低（P<0.001）。植物乳杆菌 LS/07 和菊粉使乳酸菌计数有统计学显著增加（P<0.001），大肠埃希菌数量减少（P<0.001）。这些结果表明，植物乳杆菌 LS/07 和菊粉可作为重要的营养补充剂或药用产品用于人类和动物的饮食。
