Institute of Experimental Medicine, P.J Safarik University, Slovak Republic.
Neoplasma. 2010;57(5):422-8. doi: 10.4149/neo_2010_05_422.
Diet interventions and natural bioactive supplements have now been extensively studied to reduce risks of colon cancer, which is one of the major public health problem throughout the world. The objective of our investigation was to study the effects of probiotic, prebiotic, nutritional plant extract, and plant oil on selected biochemical and immunological parameters in rats with colon cancer induced by N,N dimethylhydrazine (DMH). Male and female Wistar albino rats were were fed by a high-fat (HF) diet (10% fat in the diet) and were divided into 9 groups: Control group; PRO group - HF diet supplemented with probiotic Lactobacillus plantarum to provide 3 x 109 c.f.u. of strain/1 ml of medium; PRE group - HF diet supplemented with inulin enriched with oligofructose (2% of HF diet); HES group - HF diet supplemented with plant extract of Aesculus hippocastanum L. (1% of HF diet); OIL group - HF diet comprised Linioleum virginale (2% of HF diet); and combination of probiotic microorganisms and bioactive compounds in the groups - PRO-PRE, PRO-HES, PRO-OIL, PRE-OIL. Carcinogenesis was initiated with subcutaneous injection of DMH (20 mg/kg) two times at week interval and dietary treatments were continued for the six weeks. Application of probiotic microorganisms and bioactive compounds in all treated groups significantly decreased the activities of bacterial enzymes (p<0.001), the fecal bile acids concentration (p<0.01; p<0.001) and significantly increased serum TNFalpha level (p<0.001) in comparison to the control rats. The number of coliforms was reduced in PRO, PRO-PRE, PRO-OIL and PRE-OIL groups and significantly higher count of lactobacilli (p<0.05) was observed in PRO-PRE, PRO-OIL and PRE-OIL groups in compare with the controls. In conclusion, the results of this study indicate that probiotic microorganisms and bioactive compounds could exert a preventive effect on colon carcinogenesis induced by DMH.
饮食干预和天然生物活性补充剂已广泛研究用于降低结肠癌的风险，结肠癌是全世界主要的公共卫生问题之一。我们的研究目的是研究益生菌、益生元、营养植物提取物和植物油对 N,N-二甲基肼（DMH）诱导的结肠癌大鼠选定生化和免疫参数的影响。雄性和雌性 Wistar 白化大鼠喂食高脂肪（HF）饮食（饮食中 10%的脂肪），并分为 9 组：对照组；PRO 组 - HF 饮食补充益生菌植物乳杆菌，提供 3 x 109 c.f.u.的菌株/1 毫升培养基；PRE 组 - HF 饮食补充富含低聚果糖的菊苣（HF 饮食的 2%）；HES 组 - HF 饮食补充欧洲七叶树（Aesculus hippocastanum L.）植物提取物（HF 饮食的 1%）；OIL 组 - HF 饮食包含亚麻籽油（HF 饮食的 2%）；以及在 PRO-PRE、PRO-HES、PRO-OIL、PRE-OIL 组中补充益生菌微生物和生物活性化合物。用皮下注射 DMH（20mg/kg）两次，间隔一周启动致癌作用，并且继续进行 6 周的饮食处理。与对照组相比，在所有治疗组中应用益生菌微生物和生物活性化合物可显著降低细菌酶的活性（p<0.001）、粪便胆汁酸浓度（p<0.01；p<0.001），并显著增加血清 TNFalpha 水平（p<0.001）。在 PRO、PRO-PRE、PRO-OIL 和 PRE-OIL 组中，大肠菌群的数量减少，并且在 PRO-PRE、PRO-OIL 和 PRE-OIL 组中观察到的乳杆菌数量显著增加（p<0.05）与对照组相比。总之，这项研究的结果表明，益生菌微生物和生物活性化合物可能对 DMH 诱导的结肠癌发生具有预防作用。
