Department of Electronics and Communication Engineering, Rajalakshmi Engineering College, Thandalam-602105, Tamilnadu, India.
Materials and MEMS Laboratory, Department of Electronics and Communication Engineering, SSN College of Engineering, Kalavakkam-603110, Tamilnadu, India.
IET Nanobiotechnol. 2020 Oct;14(8):695-700. doi: 10.1049/iet-nbt.2020.0020.
A simple ultrasonic assisted chemical technique was used to synthesise cadmium oxide (CdO) nanoparticles (NPs) and CdO NPs/c-Multiwalled carbon nanotube (c-MWCNT) nanocomposite fibres.To confirm the physio-chemico properties and to analyse surface morphology of the obtained nanomaterials X-Ray Diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR) and field emission scanning electron microscopy (FESEM) were performed. To evaluate the anti-cancer property of CdO NPs, c-MWCNT NPs and CdO NPs/c-MWCNT nanocomposite fibres, an anti-proliferative assay test (Methylthiazolyl diphenyl- tetrazolium bromide - MTT assay) were performed on HeLa cells which further estimated IC50 value (Least concentration of sample in which nearly 50% of cells remain alive) under in-vitro conditions. On comparison, CdONPs/c-MWCNT based system was found to be superior by achieving 52.3% cell viability with its minimal IC50 value of 31.2 μg/ml. Lastly, the CdO NPs based system was taken up for an apoptotic study using DNA fragmentation assay for estimating its ability to cleave the DNA of the HeLa cells into internucleosomal fragments using the agarose gel electrophoresis method. In conclusion, based on our observations, CdO NPs/c-MWCNT hybrid based system can be further used for the development of efficient drug delivery and therapeutic systems.
采用简单的超声辅助化学技术合成了氧化镉（CdO）纳米粒子（NPs）和 CdO NPs/c-多壁碳纳米管（c-MWCNT）纳米复合材料纤维。为了确认获得的纳米材料的物理化学性质并分析其表面形态，进行了 X 射线衍射（XRD）、傅里叶变换红外光谱（FTIR）和场发射扫描电子显微镜（FESEM）分析。为了评估 CdO NPs、c-MWCNT NPs 和 CdO NPs/c-MWCNT 纳米复合材料纤维的抗癌性能，在 HeLa 细胞上进行了抗增殖测定试验（噻唑蓝-溴化四唑比色法（MTT 试验）），进一步在体外条件下估算了 IC50 值（几乎 50%的细胞存活的样品的最低浓度）。相比之下，CdO NPs/c-MWCNT 基体系通过实现 52.3%的细胞活力和最小的 IC50 值 31.2 μg/ml 被证明更优越。最后，使用琼脂糖凝胶电泳法对 DNA 片段化测定进行了凋亡研究，以评估 CdO NPs 基体系将 HeLa 细胞的 DNA 切割成核小体片段的能力。总之，根据我们的观察，CdO NPs/c-MWCNT 杂化基体系可进一步用于开发有效的药物输送和治疗系统。
