Institute of Molecular Biology (IMB), Ackermannweg 4, D - 55128 Mainz, Germany.
STAR Protoc. 2020 Aug 5;1(2):100076. doi: 10.1016/j.xpro.2020.100076. eCollection 2020 Sep 18.
GLOE-Seq is a next-generation sequencing method for the genome-wide mapping of 3'-OH termini, either resulting from single- or double-strand breaks or introduced by enzymatic conversion of lesions or modified nucleotides. This protocol provides instructions for isolation of genomic DNA from budding yeast or mammalian cells, preparation of libraries for sequencing, and data analysis by the associated computational pipeline, GLOE-Pipe. It is optimized for the Illumina next-generation sequencing platform and can be adapted to intact genomic DNA of any origin. For complete details on the use and execution of this protocol, please refer to Sriramachandran et al. (2020).
GLOE-Seq 是一种用于全基因组范围内检测 3'-OH 末端的新一代测序方法，这些 3'-OH 末端可以由单链或双链断裂产生，也可以由酶促转化损伤或修饰核苷酸产生。本方案提供了从芽殖酵母或哺乳动物细胞中分离基因组 DNA、制备测序文库以及通过相关计算管道 GLOE-Pipe 进行数据分析的说明。它是针对 Illumina 下一代测序平台进行了优化的，也可以适用于任何来源的完整基因组 DNA。如需详细了解本方案的使用和执行方法，请参考 Sriramachandran 等人（2020 年）的研究。
