Center of Bioinformatics, Biostatistics and Integrative Biology, Institut Pasteur - Bioinformatics and Biostatistics Hub - C3BI, USR 3756 IP CNRS, Paris, France.
Center for Technological Resources and Research, Institut Pasteur - Transcriptome and Epigenome Platform - Biomics Pole - C2RT, Paris, France.
Bioinformatics. 2019 Mar 1;35(5):901-902. doi: 10.1093/bioinformatics/bty706.
When sequencing several libraries simultaneously, the selection of compatible combinations of indexes is critical for ensuring that the sequencer will be able to decipher the short, sample-specific barcodes added to each fragment. However, researchers have few tools to help them choose optimal indexes. Here, we present checkMyIndex, an online R/Shiny application that facilitates the selection of the right indexes as a function of the experimental constraints.
checkMyIndex is available free of charge at https://checkmyindex.pasteur.fr as an online, web-based R/Shiny application. The source code is available on GitHub at https://github.com/PF2-pasteur-fr/checkMyIndex.
在同时对多个文库进行测序时，选择兼容的索引组合对于确保测序机能正确解析添加到每个片段的短的、样本特异性的条码至关重要。然而，研究人员几乎没有工具来帮助他们选择最佳索引。在这里，我们介绍了 checkMyIndex，这是一个在线 R/Shiny 应用程序，它可以根据实验约束条件方便地选择正确的索引。
checkMyIndex 可在 https://checkmyindex.pasteur.fr 上免费获得，作为一个在线的基于网络的 R/Shiny 应用程序。源代码可在 GitHub 上的 https://github.com/PF2-pasteur-fr/checkMyIndex 获得。
