Okamura Tomoharu, Chen Liji, Tsumano Nobuhito, Ikeda Chihoko, Komasa Satoshi, Tominaga Kazuya, Hashimoto Yoshiya
Department of Oral Pathology, Osaka Dental University, 8-1, Kuzuhahanazonocho, Hirakata, Osaka 573-1121, Japan.
Department of Orthodontics, Osaka Dental University, 8-1, Kuzuhahanazonocho, Hirakata, Osaka 573-1121, Japan.
Materials (Basel). 2020 Oct 26;13(21):4770. doi: 10.3390/ma13214770.
The Bio-C Sealer is a recently developed high-plasticity, calcium-silicate-based, ready-to-use material. In the present study, chemical elements of the materials were characterized using scanning electron microscopy (SEM), X-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FTIR). The biocompatibility of the Bio-C Sealer was investigated using cytotoxicity tests and histological responses in the roots of dogs' teeth. XRD, SEM, and FTIR produced hydrated calcium silicate in the presence of water molecules. In addition, FTIR showed the formation of calcium hydroxide and polyethylene glycol, a dispersing agent. The 1:4 dilutions of Bio-C Sealer presented weaker cytotoxicity than the Calcipex II in an in vitro system using the V-79 cell line. After 90 d, the periradicular tissue response of beagle dog roots was histologically evaluated. Absence of periradicular inflammation was reported in 17 of the 18 roots assessed with the Bio-C Sealer, whereas mature vertical periodontal ligament fibers were observed in the apical root ends filled with the Bio-C Sealer. Based on these results and previous investigations, the Bio-C Sealer is recommended as an effective root-end filling material. These results are relevant for clinicians considering the use of Bio-C Sealer for treating their patients.
Bio-C封闭剂是一种最近研发的具有高可塑性、基于硅酸钙的即用型材料。在本研究中，使用扫描电子显微镜（SEM）、X射线衍射（XRD）和傅里叶变换红外光谱（FTIR）对材料的化学元素进行了表征。使用细胞毒性试验和犬齿根部的组织学反应研究了Bio-C封闭剂的生物相容性。XRD、SEM和FTIR在有水分子存在的情况下生成了水合硅酸钙。此外，FTIR显示形成了氢氧化钙和作为分散剂的聚乙二醇。在使用V-79细胞系的体外系统中，Bio-C封闭剂的1:4稀释液表现出比Calcipex II更低的细胞毒性。90天后，对比格犬牙根的根尖周组织反应进行了组织学评估。在用Bio-C封闭剂评估的18个牙根中，有17个报告无根尖周炎症，而在充满Bio-C封闭剂的根尖末端观察到成熟的垂直牙周韧带纤维。基于这些结果和先前的研究，Bio-C封闭剂被推荐为一种有效的根尖充填材料。这些结果对于考虑使用Bio-C封闭剂治疗患者的临床医生具有参考价值。
