López-García Sergio, Pecci-Lloret Miguel R, Guerrero-Gironés Julia, Pecci-Lloret María P, Lozano Adrián, Llena Carmen, Rodríguez-Lozano Francisco Javier, Forner Leopoldo
Cellular Therapy and Hematopoietic Transplant Unit, Internal Medicine Department, Virgen de la Arrixaca Clinical University Hospital, IMIB-Arrixaca, University of Murcia, 30120 Murcia, Spain.
Department of Genetics and Microbiology, Faculty of Biology, University of Murcia, 30100 Murcia, Spain.
Materials (Basel). 2019 Sep 22;12(19):3087. doi: 10.3390/ma12193087.
The aim of this study was to investigate the cytocompatibility and mineralization potential of two premixed hydraulic endodontic sealers compared with an epoxy resin-based root canal sealer. The cellular responses and mineralization capacity were studied in human periodontal ligament stem cells (hPDLSCs) that were exposed to premixed hydraulic sealers, Bio-C Sealer (Angelus, Londrína, PR, Brazil), TotalFill BC Sealer (FKG Dentaire SA, La-Chaux-de-fonds, Switzerland) and an epoxy resin-based material, AH Plus (Dentsply De Trey, Konstanz, Germany). Non-exposed cultures served as the control. The endodontic sealers were assessed using scanning electron microscopy (SEM) and energy dispersive X-ray microanalysis (EDX). Statistical analyses were done using Analisis of Variance (ANOVA), with Bonferroni adjusted pairwise comparison ( = 0.05). AH Plus reduced cell viability and cell migration, whereas increased cell viability and cell migration were observed in the Bio-C Sealer and the TotalFill BC Sealer ( < 0.05). The lowest cell attachment and spreading were observed for all concentrations of AH Plus, whereas the highest were observed for TotalFill BC Sealer. At the end of 21 days, only the Bio-C Sealer and the TotalFill BC Sealer supported matrix mineralization ( < 0.05). Additionally, SEM-EDX revealed high content of calcium, oxygen, and silicon in the Bio-C Sealer and the TotalFill BC Sealer. Based on the results from this study, Bio-C Sealer and TotalFill BC Sealer demonstrated better cytocompatibility in terms of cell viability, migration, cell morphology, cell attachment, and mineralization capacity than AH Plus.
本研究的目的是调查两种预混水硬性牙髓封闭剂与一种环氧树脂基根管封闭剂相比的细胞相容性和矿化潜力。在暴露于预混水硬性封闭剂、Bio-C封闭剂（安吉利斯公司，巴西隆德里纳）、TotalFill BC封闭剂（FKG牙科公司，瑞士拉绍德封）和一种环氧树脂基材料AH Plus（登士柏德瑞公司，德国康斯坦茨）的人牙周膜干细胞（hPDLSCs）中研究细胞反应和矿化能力。未暴露的培养物作为对照。使用扫描电子显微镜（SEM）和能量色散X射线微分析（EDX）评估牙髓封闭剂。使用方差分析（ANOVA）进行统计分析，并进行Bonferroni校正的成对比较（α = 0.05）。AH Plus降低了细胞活力和细胞迁移，而在Bio-C封闭剂和TotalFill BC封闭剂中观察到细胞活力和细胞迁移增加（P < 0.05）。在所有浓度的AH Plus中观察到最低的细胞附着和铺展，而在TotalFill BC封闭剂中观察到最高的细胞附着和铺展。在21天结束时，只有Bio-C封闭剂和TotalFill BC封闭剂支持基质矿化（P < 0.05）。此外，SEM-EDX显示Bio-C封闭剂和TotalFill BC封闭剂中钙、氧和硅的含量很高。基于本研究的结果，Bio-C封闭剂和TotalFill BC封闭剂在细胞活力、迁移、细胞形态、细胞附着和矿化能力方面表现出比AH Plus更好的细胞相容性。
