结直肠癌患者血清氨基酸代谢与淋巴结转移关系的研究
Study of the Relationship Between Serum Amino Acid Metabolism and Lymph Node Metastasis in Patients with Colorectal Cancer.
作者信息
Liu Jinhao, Wang Jikun, Ma Xueqian, Feng Yang, Chen Yanlei, Wang Yanping, Xue Dong, Qiao Shifeng
机构信息
The Second Ward of General Surgery, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning, People's Republic of China.
Oncology Department, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning, People's Republic of China.
出版信息
Onco Targets Ther. 2020 Oct 13;13:10287-10296. doi: 10.2147/OTT.S273107. eCollection 2020.
PURPOSE
Lymph node metastasis is one of the important prognostic factors of colorectal cancer, and an important index of individualized treatment. The purpose of this study is to use metabonomics to identify potential molecular markers of lymph node metastasis in colorectal cancer (CRC).
PATIENTS AND METHODS
Peripheral blood samples of 223 CRC patients were collected. The metabolic levels of amino acids and carnitine in peripheral blood of CRC patients, with and without lymph node metastasis, were analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS).
RESULTS
The results show that there were significant differences in the levels of serum amino acids and carnitine between lymph node metastatic patients and lymph node non-metastatic patients. The diagnostic model that was constructed by 9 types of serum metabolites has a high diagnostic ability.
CONCLUSION
LC-MS/MS is a detection method that has a broad application in predicting CRC prognosis, individualized treatment, and in studying the mechanism of lymph node metastasis.
目的
淋巴结转移是结直肠癌重要的预后因素之一，也是个体化治疗的重要指标。本研究旨在利用代谢组学鉴定结直肠癌（CRC）淋巴结转移的潜在分子标志物。
患者与方法
收集223例CRC患者的外周血样本。采用液相色谱-串联质谱（LC-MS/MS）分析有或无淋巴结转移的CRC患者外周血中氨基酸和肉碱的代谢水平。
结果
结果显示，淋巴结转移患者与无淋巴结转移患者的血清氨基酸和肉碱水平存在显著差异。由9种血清代谢物构建的诊断模型具有较高的诊断能力。
结论
LC-MS/MS是一种在预测CRC预后、个体化治疗以及研究淋巴结转移机制方面具有广泛应用的检测方法。
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