Kimura T, Sumii T, Oogami Y, Yamaguchi H, Ibayashi H, Kinjo M
Third Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Gastroenterol Jpn. 1987 Aug;22(4):480-6. doi: 10.1007/BF02773817.
Two types of experimental chronic pancreatic damage were induced in rats by a copper-deficient diet containing D-penicillamine (DP rats) and by a similar diet without D-penicillamine (D rats). At 5-6 weeks after commencement of treatment, serum copper levels showed a significant decrease in both DP rats (1.5 +/- 0.9 micrograms/dl, M +/- SE) and D rats (18.8 +/- 1.4 micrograms/dl) compared with the findings in control rats (111.8 +/- 4.4 micrograms/dl). Histology revealed loss and atrophy of acinar cells in DP rats and mild vacuolar degeneration of acinar cells in D rats, whereas the islets of Langerhans were intact in both groups. The secretion of the exocrine pancreas showed a marked decrease in enzymes and protein output in DP and D rats compared with findings in the control rats. Our study confirmed the relationships between serum copper levels and morphological damage of pancreatic acinar cells or the reduction of enzyme secretion. On the other hand, the reductions in volume and bicarbonate output of pancreatic juice in DP rats and in D rats were detected, but no relationship was found between these reductions and the serum copper levels. The endocrine pancreatic function in DP rats showed a slight disturbance in intravenous glucose tolerance test. These results support the conclusion that copper-deficiency causes disturbances in enzyme-synthesis and selective destruction of acinar cells.
通过含D-青霉胺的缺铜饮食（DP大鼠）和不含D-青霉胺的类似饮食（D大鼠）在大鼠中诱导出两种实验性慢性胰腺损伤。在治疗开始后5 - 6周，与对照大鼠（111.8±4.4微克/分升）的结果相比，DP大鼠（1.5±0.9微克/分升，均值±标准误）和D大鼠（18.8±1.4微克/分升）的血清铜水平均显著降低。组织学检查显示，DP大鼠的腺泡细胞丢失和萎缩，D大鼠的腺泡细胞有轻度空泡变性，而两组的朗格汉斯岛均完整。与对照大鼠的结果相比，DP和D大鼠的外分泌胰腺分泌的酶和蛋白质输出显著减少。我们的研究证实了血清铜水平与胰腺腺泡细胞形态损伤或酶分泌减少之间的关系。另一方面，检测到DP大鼠和D大鼠的胰液体积和碳酸氢盐输出减少，但这些减少与血清铜水平之间未发现关联。DP大鼠的内分泌胰腺功能在静脉葡萄糖耐量试验中显示出轻微紊乱。这些结果支持了缺铜会导致酶合成紊乱和腺泡细胞选择性破坏这一结论。
